Multisizer 4e Coulter Counter

Synthetic biology is driving a new era in sustainable science—enabling researchers to design microorganisms for the efficient production of environmentally friendly materials. Whether you're engineering microbial strains or optimizing growth conditions, minimizing waste and maximizing process efficiency are core goals of any green chemistry workflow.

Download this eBook to:

• Miniaturize and automate strain development — cut reagent use and reduce plastic waste
• Improve strain growth and optimization — minimize costly trial-and-error steps
• Speed up process development — reduce timelines without sacrificing data quality

Explore real-world case studies and practical tools that support sustainable innovation—from DNA purification to high-throughput bioprocessing. Learn how automation, precision liquid handling, and scalable workflows can help you bring green chemistry principles to life in your lab.

Resource details:

• Document type: SelectScience guide
• Page count: 91
• Read time: 2.5 hours
• Edition: 1st

Escherichia coli (E. coli) is a facultative anaerobic bacterium, easily cultured in labs, making it a key model organism in genetics, microbiology, and biotechnology. It is commonly used for large-scale production of therapeutic proteins, with 30% of approved therapeutic proteins produced using E. coli. Beckman Coulter Life Sciences demonstrates the use of the BioLector microbioreactor and the Multisizer 4e Coulter Counter to optimize E. coli culture conditions and characterize cell growth. The BioLector is small-scale, automated system that enables high-throughput screening, cultivation parameter monitoring and feeding strategy optimization. The Multisizer 4e is an accurate and flexible particle characterization device with a sizing range of 0.2–1600 μm. Generated data are processed using patented digital pulse processing technology and can be saved and later re-analyzed.

Whether your cells are bacteria, animal cells or plankton, this white paper discusses how the Coulter Principle is one of the most universal and adaptable techniques for studying cell size or simply counting cells. It has been a stable and proven technology since it was developed in the late 1940’s. In this guide a number of methodologies utilizing the Coulter Principle are presented together with some typical examples.

Beckman Coulter’s Multisizer 4 provides analysts with an easy-to-use, technologically-advanced system that can solve most particle sizing and counting challenges. Although the procedures described in this application note address common issues encountered when analyzing subvisible particles in protein formulations, the general guidelines can also be used for applications requiring small aperture tubes.

The Coulter Principle has become the accepted "Reference Method" throughout the world for particle size analysis. This white paper is a short course in the Coulter Principle and particle characterization using the Multisizer and Z-series instruments.

Lena Lee discusses the latest Multisizer 4e COULTER COUNTER from Beckman Coulter. The new COULTER COUNTER features a 10-micron aperture enabling users to obtain accurate count, size and mass distribution for particles and cells ranging from 0.2 to 1,600 microns. Digital Pulse Processing provides high-resolution analysis in as many as 400 channels for unparalleled performance in pharmaceutical quality control, bioprocessing and industrial applications.

Learn about the six steps of CAR T cell production and the technology ensuring optimal cell delivery to patients

Scientists share how automated cell counting is revolutionizing research