Microglia Phagocytosis Assay Service
Microglia are the resident macrophages in the central nervous system (CNS). One of their most important characteristics is their ability to phagocytose, which plays a pivotal role in maintaining brain homeostasis and responding to neurodegenerative diseases. In vitro microglial phagocytosis assays assist in understanding how microglia maintain tissue homeostasis in healthy and disease states.
Microglia Phagocytosis Assay Service
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Our optimized physiological culture method accurately reproduces the characteristics and morphology of microglia in vivo. Additionally, our laboratory is equipped with a live cell imaging device designed to effectively capture the cellular changes that occur throughout the life cycle of microglia, enabling long-term measurements and real-time cell visualization.
Microglia Phagocytosis Assays Methodology
Fluorescence-labeled microspheres or bioparticles: Phagocytosis experiments typically utilize fluorescently labeled microspheres or bioparticles as phagocytic targets. In a typical assay, microglia are treated, then phagocytic targets are diluted and introduced to microglial culture medium, after which phagocytic activity is evaluated through immunofluorescence staining or flow cytometry.
pH-dependent fluorescent conjugate: We use a range of pH-dependent fluorescent conjugates, which are pH-sensitive dye conjugates that fluoresce brightly in phagosomes and can label proteins, beta-amyloid (1-42), α-synuclein, and dead cells, making the assay highly customizable. The fluorescence of the assay increases as the pH decreases. No washing or quenching steps are required, and no compensation for environmental factors is needed, ensuring faster and more accurate results.
At Creative Biolabs, our microglial phagocytosis assay service employs cutting-edge technology to efficiently and gently isolate microglia and analyze microglial phagosomes, elucidating their pivotal roles in brain health and disease. This service offers invaluable support for neuroscience research. We invite you to contact us to discuss your project plans in detail.
