JumpStart™ Taq ReadyMix™
Complete optimized reagent for hot-start PCR at 2X concentration
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JumpStart™ Taq ReadyMix has been used in quantitative polymerase chain reaction (PCR). It has also been used to identify fibroblast cell surface adenosine receptor (AR) types under hypoxia.
One-Step RT-qPCR products for high-throughput research applications
At each cycle during PCR, the quantity of DNA is measured in real time by using a variety of fluorescent chemistries. The most common approaches to generate a fluorescent signal are to use either hydrolysis probes such as TaqMan® probes, or a double-stranded DNA binding dye such as SYBR® Green dye. The selection of fluorescent chemistry is dependent upon factors such as the application, cost, and whether the assay is a singleplex or multiplex assay. DNA-binding dyes are preferred for singleplex, low-throughput assays since they are easier to design, have lesser setup time, and are more cost efficient. Fluorescent probes are more commonly employed in high-throughput, multiplex assays that require higher specificity.
