Invitrogen™ TrueCut™ HiFi Cas9 Protein
High fidelity Cas9 protein engineered to significantly reduce off-target effects while maintaining high editing efficiency
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TrueCut HiFi Cas9 Protein is a high fidelity CRISPR Cas9 protein variant engineered to demonstrate superior off-target profiles, while preserving maximum on-target editing efficiency. TrueCut HiFi Cas9 Protein is ideal for applications that are sensitive to off-target events and when more precise editing is required.
Features include:
- Near complete elimination of off-target events in all tested cell lines including standard, immune, primary, and stem
- Maintains maximum on-target editing efficiency compared to TrueCut Cas9 Protein v2
- High quality—manufactured under strict ISO 13485 quality standards
- Transfection-ready, using either electroporation or lipid-mediated transfection reagents
- Eliminates time-consuming cloning steps
Cas9 plasmids vs proteins for CRISPR gene editing experiments
CRISPR-Cas9 systems provide a platform for direct and high-efficiency modification of genomic DNA. However, the delivery of Cas9 and synthesis of guide RNA (gRNA) remain limiting factors in the gene editing workflow.
Cas9 proteins are gaining popularity due to their promising predictability, efficiency, and robustness in delivery and editing, along with overall ease of use and time savings.
In this infographic, we outline how your CRISPR gene editing experiments can benefit from Cas9 proteins, including:
A comparison of the Cas9 plasmid vs protein workflows
The 5 key benefits of using Cas9 proteins>
A suite of next-generation Cas9 protein products >
For further information, find out how to design your CRISPR gene editing experiments in as little as five minutes with this expert article.
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