Gilson PIPETMAX

In this application note, Gilson introduces an automated method for the Agencourt® AMPure® XP PCR purification system using PIPETMAX®. This method simplifies and enhances the reliability and flexibility of a typically laborious and technically challenging process. Genomic DNA from Escherichia coli was prepared for whole genome sequencing using the Illumina® Nextera® XT DNA Library Preparation Kit. The Agencourt AMPure XP PCR purification system is employed to clean up the library post-amplification and before normalization. A custom magnetic bead separator rack allows efficient purification of up to 96 samples in a single run. The automated protocol is adjustable, accommodating various factors like sample quantity, volume, bead volume, wash steps, and incubation times to suit specific application needs. This application enables seamless integration of PIPETMAX into diverse workflows.

qPCR is a very versatile technique with many applications, however, it is also very sensitive and therefore requires accuracy and precision in liquid handling in order to reduce technical errors, cross-contamination, and yield reliable data.

In this free eBook, we present a number of high-throughput sample preparation methods for synthetic and molecular biology applications, and explore how automation in liquid handling can transform the throughput, accuracy, and reliability of qPCR and nucleic acid normalization in different laboratory workflows.

Download your free copy of the eBook to discover solutions for:

• Ensuring sample integrity in qPCR
• Optimizing qPCR master mix dispensing
• RNA normalization for qPCR
• DNA normalization for qPCR
• Streamlining plant RNA extraction for RT-qPCR
• Standardizing RT-qPCR workflows for SARS-CoV-2
  

When developing novel kinase inhibitors for therapeutic use, kinase profiling is required not only to confirm target selectivity, but to assess off-target activity as well. Yet, optimizing kinase reactions is a particularly arduous task. This poster illustrates the use of ready-to-use kinase selectivity profiling systems for easy, in-house kinase analysis.

CRISPR/Cas9 technology is a cutting-edge gene-engineering tool, that allows the rapid and precise editing of genetic sequences in a wide range of organisms. As with many techniques in modern molecular biology, CRISPR/ Cas9 workflows are dominated by pipetting, liquid handling and the complexing and delivery of key reagents. This application note demonstrates how Gilson products enable the high level of accuracy and precision required for all levels of a CRISPR/Cas experimental workflow.

Learn how the PIPETMAX® personal liquid handling system from Gilson, Inc. automates an assay for the identification of mutations in the coding regions of the BRCA1 and BRCA2 genes.

Cross-contamination is a common concern when a laboratory contemplates moving towards automating an assay. The PIPETMAX with qPCR Assistant is a highly proficient pipetting workstation that reduces the potential for cross-contamination and other human induced errors. This application note describes the features of the PIPETMAX and how to use it.

The techniques of qPCR and RT-qPCR have grown in popularity because of the analytical power they offer; however, there are numerous steps and components that can lead to errors. Download this guide for advice on avoiding errors during your experiment.

Normalization of the concentration of nucleic acid samples is a crucial yet tedious process that requires constant readjustment of the volume of the pipetting device. PIPETMAX® Normalization Assistant automates this process: calculations are carried out by the software, eliminating arithmetic errors, and pipetting volumes are adjusted automatically. Users can import and export sample information and reports, improving the traceability of the normalization workflow. This application note demonstrates the normalization of three examples of nucleic acids using Normalization Assistant: genomic DNA, cDNA, and RNA.

Oxidative stress plays a role in the development as well as the degeneration of the brain. Oxygen levels in the developing embryo are low and proper regulation of oxygen levels is crucial for stem cells, neural progenitor cells and neurons. This poster investigates the role of oxidative stress in human induced pluripotent stem cells (iPS cells) by testing expression of several genes involved in the cellular oxidative stress response when cells were maintained in different oxygen concentrations.

Normalization of RNA concentration before reverse transcription reduces error in qPCR experiments and is recommended by MIQE guidelines. This application note demonstrate how the PIPETMAX Normalization Assistant and PIPETMAX qPCR Assistant provide an automated, powerful, easy-to-use system that can integrate into your gene expression analysis workflow to provide reliable results, while eliminating bookkeeping and user variation.

Gilson and Funai are excited to announce the launch of the Gilson Digital Dispenser this year. This innovative new offering is a non-contact dispenser capable of dispensing DMSO and aqueous solutions from pL to uL. The large dynamic range makes it ideal for dose-response studies, serial dilutions, and assay miniaturization.

In this SelectScience webinar, our attending experts will demonstrate the functionality of the new Gilson Digital Dispenser with a cell-based assay example. During which, HEK 293 cells were dosed with doxorubicin from 0.00032 to 10 µM concentrations, and viability at 48 hours was quantified using Promega CellTiter-Glo® Luminescent Cell Viability Assay.

Key learning objectives:

• Explore affordable digital dispensing solutions from Gilson and Funai
• Discover how these dispensing solutions can be used within a cell-based assay

Who should attend:

Laboratory managers, laboratory technicians, scientists and students

In this video, Gilson, Inc. introduces the PIPETMAX®​​​​​​, an automated pipetting solution for the efficient processing of high-throughput biological assays. This promises to help you improve the accuracy, reproducibility, and consistency among all samples processed. Unlike traditional automation platforms, PIPETMAX comes in a benchtop size that easily fits into any lab.

The Normalization Assistant allows everyone in your lab to create personalized fully automated DNA or RNA normalization protocols. Bring effortless traceability and reliability to your DNA or RNA normalization sample prep while the Normalization Assistant performs all of your normalization sample prep for you, with no programming knowledge required. With the Normalization Assistant, you can quickly perform QC checks, import and export sample information to save time and prevent errors and speed up sample analysis.

Manual sample preparation can often be inefficient, complex and time-consuming. This leads to increased training requirements, preparation time, procedural errors and operational costs. PIPETMAX, from Gilson, is an easy-to-use, automated liquid handling platform than can solve these problems with automated sample preparation solutions. Unlike traditional automation platforms, PIPETMAX comes at a size and price that is accessible to any lab, and has a PIPETMANinside!

Manual sample preparation can often be inefficient, complex and time-consuming. This leads to increased training requirements, preparation time, procedural errors and operational costs. Watch this video to discover the new PIPETMAX, from Gilson, an easy-to-use, automated liquid handling platform than can solve these problems with automated sample preparation solutions. Learn about the unique features, flexibility and benefits of the new PIPETMAX.