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DNeasy 96 PowerSoil Pro QIAcube HT Kit (5)

For the isolation of microbial genomic DNA from soil and stool on the QIAcube HT

QIAGEN

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Good results, high performance, good reproduce ability

 

Average Rating 4.0

Application Area:

DNA

Great performance

Review Date: 22 Oct 2022 | QIAGEN

Extracting microbial DNA from large amounts of soil and stool samples can be challenging. QIAGEN’s new DNeasy 96 PowerSoil Pro QIAcube HT Kit uses our new Pro technology to enable isolation of high yields of pure microbial DNA from all soil types, including compost, clay and top soil, stool and gut samples. The kit is automated on the QIAcube HT for reproducible purification of up to 96 samples at one time.


The new PowerBead Pro Tubes or PowerBead Pro Plates can be purchased separately for homogenization in the preferred format. The beads and optimized chemistry enable more efficient lysis of bacteria and fungi. The kit also contains streamlined Inhibitor Removal Technology (IRT) to eliminate the challenging inhibitors commonly found in stool, soil and environmental samples in even less time. Sequencing results reveal higher alpha diversity as measured by observed operational taxonomic units (OTUs) compared to other tested methods.

Application NoteLife Sciences

Extraction of Microbiome DNA Using the DNeasy PowerSoil Pro Kit Manually and Automated on the QIAcube Connect and QIAcube

With our increasing understanding of the microbial world, it becomes clear that microbial communities play an important role in health and the environment. Analyzing microbiomes is challenging. Many sample types, including stool and soil, contain substances that inhibit downstream analyses such as PCR or Next-generation sequencing (NGS). This inhibition can bias results as some analytes might be more affected than others. Here, we show that microbiome analysis on QIAcube Connect and QIAcube ensures unbiased and efficient analysis of the microbiome. Both instruments use proven silica-membrane technology for sample processing, ensuring standardization for research labs and high-quality results.

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