COVID-19 Rapid Isothermal PCR Kit
This PCR test kit utilizes the RT-LAMP (reverse transcription loop-mediated isothermal amplification) method for the qualitative detection of SARS-CoV-2 in purified RNA isolated from nasopharyngeal swabs. Results detected by eye or plate reader.
Place your order directly with the manufacturer.
Antigen InformationProtein Name & Synonyms:
Gene encoding for the Nucleocapsid (N) protein
Assay FormatSensitivity:
25 viral RNA copies / µL
Compatible Sample Types:
Extracted RNA (e.g., from a throat swab)
Method of Detection:
RT-LAMP
Quantitative/Semi-Quantitative:
Qualitative (by eye) & Semi-Quantitative (with plate reader)
Product SpecificationsSize:
25 or 100 tests (tubes)
Number of Targets Detected:
6 regions specific to the SARS-CoV-2 N gene. The regions selected for amplification do not share homology to nucleocapsid genes from any other coronaviruses.
Plate Reader Compatibility:
Absorbance at 570 nm and 650 nm
RT-LAMP Introduction
This assay utilizes reverse transcription loop-mediated isothermal amplification (RT-LAMP), which amplifies SARS-CoV-2 RNA without the need for thermal cycling. This is accomplished using specialized primers that enable continuous amplification of nucleic acids at 60°C. RT-LAMP is faster than traditional RT-PCR with a colorimetric endpoint readout. Thus, detection of viral RNA can be performed by eye or with a plate reader capable of reading absorbance at 570 nm and 650 nm.
This kit uses six primer-probe sets specific to the SARS-CoV-2 gene encoding for the nucleocapsid protein. The regions selected for amplification do not share homology to nucleocapsid genes from any other coronaviruses.
Absence of viral RNA is represented by a purple color (570 nm)(see picture above). The presence of viral RNA is represented by a blue color (650 nm). An OD ratio of 650 nm / 570 more than 1.0 indicates that SARS-CoV-2 viral RNA is present.
Kit ComponentsComponent25 Tests100 TestsRayBio® COVID-19 Rapid Master Mix375 µl1.50 mlSARS-CoV-2 Primer Mix125 µl500 µlSARS-CoV-2 Synthetic Nucleocapsid RNA (1000 copies/µl)10 µl10 µlUltra-pure DNase- and RNase-free Water1 ml1 mlManual11
This kit also has an option to include PCR tubes. 25 tests = 4 strips of 8 PCR tubes per strip. 100 tests = 13 strips of 8 PCR tubes per strip.
Features
- Rapid: Detect SARS-CoV-2 nucleic acid in 30 minutes
- Affordable: No expensive PCR instrument required!
- Easy to use: Read results by eye
- Sensitive: Detects as few as 25 copies/µL of SARS-CoV-2 virus
- Stable: Contains reagents to reduce DNA carry-over contamination. Also pH-independent.
