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C2MAP™ System

LC/MS/MS cell culture media analysis platform

Shimadzu Corporation

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Description

The C2MAP™ System is a cell culture media analysis platform that uses liquid chromatography with tandem mass spectrometry (LC/MS/MS) to profile temporal changes in up to 95 culture supernatant components. It supports animal cell culture optimization by revealing media component consumption and depletion alongside changes in metabolites secreted by cells.

Automated culture supernatant analysis

The C2MAP™ System automates the workflow from sample pre-treatment through measurement, reducing manual steps when analyzing culture supernatant samples. Its optimized simultaneous LC/MS/MS analysis method enables multiple media components to be monitored during culturing.

Visualization of temporal changes

Measurement results are displayed as graphs, making component variations over time easier to assess. These temporal profiles help researchers understand how culture media composition changes as cells consume nutrients and release metabolites.

Key features and benefits of C2MAP System:

  • Profiles temporal changes for up to 95 culture supernatant components
  • Automates sample pre-treatment and measurement processes
  • Uses an optimized LC/MS/MS simultaneous analysis method
  • Graphically displays component changes throughout cell culturing
  • Supports animal cell culture condition optimization through media and metabolite monitoring

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