BalanCD HEK293
BalanCD™ HEK293 is a chemically defined, animal component-free medium optimized for HEK293 cell growth, transfection, viral vector production, and recombinant protein expression. Designed for scalability and workflow simplicity, it supports gene therapy development, transient gene expression, and protein production from research through commercial manufacturing.
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BalanCD™ HEK293 is a versatile, chemically defined, animal component-free medium engineered to support the growth, transfection, and production capabilities of HEK293 cell lines in suspension culture. Optimized for transient gene expression, viral vector production, and recombinant protein expression, it provides researchers and bioprocess developers with a robust platform for accelerating cell culture workflows and maximizing productivity.
The medium is designed to support a wide range of applications, including adeno-associated virus (AAV) production, lentivirus (LV) production, gene therapy development, and recombinant protein production. Its flexible formulation enables the use of a single medium throughout multiple process stages, including cell expansion, seeding, transfection, and production, helping simplify process development and reduce operational complexity.
As part of the BalanCD HEK293 System, the medium can be paired with BalanCD™ HEK293 Feed for transient protein expression or paired with BalanCD™ HEK293 Viral Feed for viral vector production. The system is compatible with flasks, spinner flasks, and bioreactors, providing a scalable solution that supports seamless transition from laboratory-scale research to commercial manufacturing.
BalanCD HEK293 is manufactured to support the demanding requirements of biopharmaceutical and gene therapy applications. Its chemically defined, animal component-free formulation helps reduce variability, while a Drug Master File (DMF) filed with the U.S. FDA supports regulatory compliance and streamlined product development.
Key Features & Benefits
- Chemically defined, animal component-free formulation
- Optimized for HEK293 suspension cell cultures
- Supports transfection, cell growth, and high-productivity cultures
- Enables viral vector production for gene therapy applications
- Supports transient protein expression and recombinant protein production
- Facilitates AAV and lentiviral vector manufacturing
- Streamlines workflows using a single medium for growth, transfection, and production
- Compatible with flasks, spinner flasks, and bioreactors
- Can be adapted from adherent to suspension HEK293 cell cultures
- Scalable from research through commercial manufacturing
- Can be combined with BalanCD HEK293 Feed or BalanCD HEK293 Viral Feed to maximize performance
- Can be utilize with Anti-Clumping Supplement to reduce cell aggregation for improved cell growth and productivity
- Drug Master File (DMF) filed with the U.S. FDA
- Designed for consistent, reproducible bioprocess performance
Applications
- HEK293 cell culture
- Viral vector production
- Gene therapy development and manufacturing
- Adeno-associated virus (AAV) production
- Lentiviral vector (LV) production
- Transient gene expression
- Transient transfection workflows
- Recombinant protein production
- Bioprocess development and optimization
- Cell and gene therapy manufacturing
- Process scale-up and technology transfer
- Commercial biopharmaceutical production
