Pall Corporation, a global leader in filtration, separation and purification, has introduced the versatile Nucleic Acid Binding (NAB) device for applications requiring highly efficient nucleic acid isolation. The NAB Nanosep® device is said to provide increased binding capacity through its innovative dual-layer, silica-based quartz glass fiber membrane.
Using the multipurpose NAB Nanosep device, researchers can purify plasmid DNA, genomic DNA, or total RNA from a variety of starting materials. The centrifugal device can recover from 10,000 to 50 base pairs, allowing users to analyze the widest range of nucleic acid fragments in a single spin device.
The NAB Nanosep device offers flexibility and rapid processing, without sacrificing yield or quality of the nucleic acids. The device’s double-layer membrane can isolate nucleic acids with yields high enough to allow multiple downstream applications, saving time and money by eliminating the need to process additional samples. Ultrasonically welded seals prevent bypass contamination or seal failure. The device fits standard centrifuge rotors that accept 1.5 mL tubes.
The NAB Nanosep device is ideal for applications in the following workflows:
Purified plasmid DNA: restriction digest, cloning, and Sanger sequencing
Purified genomic DNA: qPCR and next-generation sequencing
Purified total RNA: RT-PCR and cDNA library construction
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