New Chemistry Offerings at Pittcon 2007
1 Mar 2007New columns from Waters Corporation were displayed at Pittcon 2007. The new range of HPLC and UPLC® columns offer many distinct advantages to separation scientists. All of the new columns are described in Waters’ brand new 2007 Chromatography Columns, Waters Quality Parts® and supplies catalog (order #720001941EN).
ACQUITY® UPLC HSS T3 Columns for UPLC Separations
The ACQUITY UPLC HSS T3 columns feature new 1.8 µm high-strength silica (HSS) particles designed specifically for UPLC applications. These new columns contain the first ultra-high purity silica-based particles optimized for routine use at pressures up to 15,000 psi (1,000 bar).
The first bonded phase to be introduced, HSS T3 is aimed at separating water-soluble, polar organic molecules via reversed-phase UPLC technology in pharmaceutical and fine chemical applications. This is an important expansion of the choice of selectivity available to scientists routinely utilizing UPLC column technology.
The ACQUITY UPLC HSS T3 columns join the ACQUITY UPLC BEH Technology™ column family which includes the C18, C8, Shield RP18, Phenyl and HILIC chemistries, all of which are designed and tested for use with Waters ACQUITY UPLC systems. ACQUITY UPLC columns are available in 1.0 and 2.1 mm internal diameters (ID) with lengths from 30 to 150 mm. For more information visit www.waters.com/acquitycolumns.
Atlantis® T3 Columns for HPLC Separations
Like ACQUITY UPLC HSS T3 columns, the new Atlantis T3 HPLC columns feature T3 bonding technology, the product of over 30 years of bonded phase research at Waters. T3 bonding combines enhanced polar compound retention with longer column lifetimes at low pH and improved peak shape for amine-containing bases at pH 7. T3 bonding also provides aqueous mobile phase compatibility and ultra-low MS bleed. For more information visit www.waters.com/polar.
Even though the ACQUITY UPLC HSS T3 columns and Atlantis T3 HPLC columns are built on different particle substrates, methods can be moved from HPLC to UPLC since both LC columns utilize the same T3 bonding and endcapping process. Atlantis T3 HPLC columns are available in 3, 5 and 10 micron particle sizes in various lengths and dimensions.
Peptide Separation Technology Columns
The new Peptide Separation Technology columns for proteomics applications, peptide mapping during the characterization of biopharmaceuticals and the purification of synthetic peptides are based on Waters’ proprietary C 18 BEH Technology particle platform.
Peptide Separation Technology columns are available in nanoLC™, HPLC and UPLC formats and are available in 1.7 µm – 10 µm particle size range, lengths from 50 to 250 mm and internal diameters (ID) of 75 µm to 30 mm. Nominal particle pore sizes are either 130 or 300Å.
The new Peptide Separation Technology columns, in combination with all Waters HPLC Systems, ACQUITY UPLC and nanoACQUITY UPLC instruments, offer scientists many benefits including improved separation of protein digests, significantly improved peptide mapping, efficient glycopeptide separations, and the ability to quantify trace peptide variants in protein biopharmaceuticals. These columns are also the first columns to feature QC procedures targeted for large molecule use. For more information visit www.waters.com/pst.
Oligonucleotide Separations Technology
ACQUITY Oligonucleotide Separation Technology (OST) C18 Columns are specifically designed for the purification and analysis of oligonucleotides. These products are ideal for purifying and analyzing synthetic oligonucleotides including siRNA, primers and probes, therapeutic drugs made by oligonucleotide manufacturers, diagnostic kit makers, government and university laboratories, and biotechnology companies.
They are available in both HPLC and UPLC formats. The XBridge™ OST C18 columns are available in 2.5 µm particle size for HPLC use, while the ACQUITY BEH 1.7µm particles are available in the higher-pressure-rated UPLC column formats.
All the columns feature BEH Technology particles and yield significantly better resolution for ion-pair, reversed-phase separations of oligonucleotides than do alternative traditional columns. Lifetime expectancy of the new columns is greater than 1,000 injections.
Amino Acid Analysis Columns
Waters UPLC Amino Acid Analysis columns are designed for determining amino-acid composition of proteins, cell culture media monitoring, and food and feed nutritional analysis. These AccQ-Tag Ultra™ columns are designed specifically for use with the Waters ACQUITY UPLC System and offer scientists sharper, better-resolved peaks, with up to five times faster separations. The columns are available immediately and are part of Application Kits containing associated AccQ-Tag™ Ultra Reagents, mobile-phase eluents, and methodologies. For more information visit www.waters.com/aaa.
VanGuard™ Pre-Columns for UPLC Separations
VanGuard Pre-Columns for UPLC separations are guard columns that are designed for use with the ACQUITY UltraPerformance LC system. These guard columns use an ultra-low volume design (patent pending) that efficiently protects UPLC column performance. This ultra-low volume design does not compromise the UPLC holistic design approach to higher efficiency, greater resolution and increase throughput. Guard columns are typically used in applications where complex, unpredictable and demanding samples are analyzed. VanGuard Pre-Columns are the first guard columns specifically designed for routine use at pressures up to 15,000 psi (1,000 bar). The first VanGuard Pre-Columns for UPLC separations will be available with 1.7 µm ACQUITY UPLC BEH C18 chemistry on April 15, 2007. Additional VanGuard Pre-Columns will be released as they become available in 2007.
Related Products
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XBridge® and ACQUITY UPLC® Oligonucleotide C18 Columns
WatersXBridge® and ACQUITY UPLC® Oligonucleotide C18 Columns for Synthetic Oligonucleotide Analysis and PurificationIon-pair, reversed-phase separation of detritylated oligonucleotides on sub-3 micron particles yields exceptional component resolution.UPLC ® technology increases sample throughput while maintaining necessary oligonucleotide component resolution.LC/UV and LC/MS methods for enhanced quantitative and qualitative oligonucleotides analyses.Various XBridge™ Oligonucleotide C 18 column dimensions address lab scale purification needs.2nd generation hybrid-silica BEH Technology™ particles deliver exceptional column life.Use of synthetic oligonucleotides for research, diagnostic, and therapeutic applications continues to increase. The availability of 2.5 μm HPLC or 1.7 μm UPLC ® technology particles in several column dimensions provides flexibility to meet various LC/UV or LC/MS application needs. Waters' stringent manufacturing and quality control procedures help ensure consistent batch-to-batch and column-to-column performance.
ACQUITY UPLC HSS T3 Columns for Polar Compound Retention
WatersACQUITY UPLC® HSS (High Strength Silica) T3 Columns contain the first and only 100% silica particle designed, tested and intended for use in applications up to 15000 psi/1000 bar. The ACQUITY UPLC® HSS particle is not an HPLC particle. High pore volume HPLC particles do not possess the mechanical stability necessary to withstand the high column packing and operating pressures of UPLC® separations. The first ligand chemistry in this UPLC®-certified column family utilizes T3 bonding in order to retain and separate polar organic compounds. ACQUITY UPLC® HSS T3 columns possess the superior polar-compound retention, aqueous mobile phase compatibility and ultra-low MS bleed of Atlantis® T3 columns. Easier Method Transfer Since Atlantis® T3 HPLC and ACQUITY UPLC® HSS T3 columns utilize T3 bonding, LC methods can be transferred from HPLC to UPLC® separations. Because Atlantis® and HSS particles are different substrates, some method modification and/or optimization may be required. A compelling benefit of UPLC® technology is the ease and speed at which this method optimization can occur since UPLC® separations offer greater resolution in less time. Moving from XBridge™ HPLC to ACQUITY UPLC® BEH columns is nearly seamless, as the hybrid particle substrates differ only in particle size. However, because of T3 bonding it should be straightforward to make simple adjustments to successfully transfer a separation from an Atlantis® T3 HPLC column to an ACQUITY UPLC® HSS T3 column.
Atlantis T3 HPLC columns
WatersThe Atlantis® T3 HPLC columns set the standard for retention and separation of polar compounds via reversed-phase HPLC. Atlantis® T3 columns are a universal, silica-based, reversed-phase C18 line of HPLC columns that not only retain and separate small, water-soluble polar organic compounds, but also provide superior performance across a wider pH range. Atlantis® columns are the culmination of over 30 years of bonded phase research and are a vast improvement upon the highly successful Atlantis® dC18 line of columns. For reversed-phase applications, Atlantis® T3 columns should be considered the first choice when developing a separation of polar (and non-polar) compounds. The advantages of T3 bonding technology are extended to UPLC® in the ACQUITY® UPLC® HSS T3 columns. T3 Bonding The superior performance of Atlantis® T3 HPLC columns and the ACQUITY® UPLC® HSS T3 columns is a result of Waters’ , advanced T3 bonding process. T3 bonding utilizes a trifunctional C18 alkyl phase bonded at a ligand density that promotes polar compound retention and aqueous mobile phase compatibility. The proprietary T3 endcapping process is much more effective than traditional trimethyl silane (TMS) endcapping. The unique combination of bonding and endcapping provides superior polar compound retention and aqueous compatibility while also enhancing column performance, lifetime, peak shape and stability. A Universal Reversed-Phase Polar Compound Retention Column Large organizations are looking to narrow or limit the number of column choices available to their separations scientists. This worldwide LC column “harmonization” can provide more predictable, controlled consumable costs, simplified method transfer between laboratory locations around the world, and pre-screened LC columns for various performance parameters. With good retention of, and selectivity for, neutral, hydrophilic compounds as well as polar analytes and superior performance over a wider pH range, Atlantis® T3 columns merit serious consideration as a more universal alternative to traditional C18 reversed-phase LC columns.