INTEGRA products improve pipetting of non-aqueous liquids
26 May 2021
INTEGRA Biosciences is dedicated to developing innovative solutions that improve liquid handling for all, including applications using non-aqueous liquids.
These can be challenging to pipette accurately, as they are commonly viscous or volatile, or have other characteristics that make them difficult to handle. INTEGRA has tested the most common non-aqueous solutions using its VIAFLO electronic pipettes, providing users with recommendations for the best tips and optimal settings to improve pipetting of liquids such as DMSO, glycerol, ethanol and Tween® 20.
Experiments suggests that use of reverse pipetting – aspirating more liquid than you need, then discarding the excess after dispensing – greatly improves pipetting of these solutions, increasing reproducibility by minimizing the influence of effects such as evaporation in the tip, attachment of liquids to the tip wall, or air bubble formation in the sample. In addition, it is vital to adjust pipetting speeds to the liquid to improve precision. The aspiration/dispense speed of the VIALFO can be set to eliminate the variability associated with manual pipettes. It is also important to use the correct tips when pipetting these challenging samples, and INTEGRA offers its GripTip pipette tips in both low retention and wide bore formats to aid pipetting of viscous liquids. By following these suggestions, users can set up their pipettes faster, requiring less testing to achieve optimal results.
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VIAFLO Electronic Pipettes
INTEGRA BiosciencesElectronic pipettes with touch wheel user interface. VIAFLO pipettes accommodate any pipetting task at hand: from single transfers to complex protocols. An intuitive touch wheel interface and color display allows for the quickest volume changes and user friendly menu navigation. The ergonomic design enables effortless pipetting. The unique tip fitting of the VIAFLO pipette assures that GripTip pipette tips will never loosen, leak or fall off. VIAFLO Electronic Pipettes Features: GripTip Pipette Tips - By designing our GripTips™ in tandem with VIAFLO pipettes, we have created a unique, integrated system that offers the securest pipette and pipette tip connection. High lateral resistance. This prevents tips from falling off when touching off a multichannel pipette within a 96 or 384 well plate. Low required tip attachment forces. Low required tip ejection forces. All tips seal at the same height ensuring a perfect touch off and increased precision when dispensing. Intuitive User Interface - A full color screen and a proven touch wheel interface offer quick and comprehensible menu navigation. Simply by moving a finger over the touch wheel, menus and settings can be accessed. All menus are spelled out on the clear to read screen making memorizing of abbreviations unnecessary. Convenient Pipette and Program Management - VIAFLO pipettes can be connected to a computer via USB port. With the free to use pipette management software VIALINK, custom programs can be created on the computer and then be transferred onto VIAFLO pipettes. For laboratories with many users or power users with many pipetting protocols, all custom programs can be stored and backed up in a library on the computer. Applications: Repeat Dispense Manual Pipetting Sample Dilution Reverse Pipetting Variable Dispense