Human/Mouse Embryonic Stem Cell 4-Color Flow Cytometry Kit

16 Mar 2009
Nadine Davies
Marketing / Sales

R&D Systems now offers a Human/Mouse Embryonic Stem Cell 4-Color Flow Cytometry Kit (Catalog # FMC001).

This kit contains four different fluorochrome-conjugated antibodies and the corresponding isotype controls for single-step staining of human and mouse embryonic stem cells. The antibodies included are PE-conjugated anti-SOX2, APC-conjugated anti-Oct-3/4, PerCP-conjugated anti-SSEA-1, and CFS-conjugated anti-SSEA-4. In addition, the kit includes fixation, permeabilization, and wash buffers.

The figure displays a Multi-color Analysis of Mouse Embryonic Stem Cells by Flow Cytometry. D3 mouse embryonic stem cells were stained with the indicated antibodies (filled histograms) or corresponding isotype controls (open histograms) provided in the Human/Mouse Embryonic Stem Cell 4-Color Flow Cytometry Kit (Catalog # FMC001).

For more information on this and other products for stem cell research, please visit our website by following the 'Company Website' link or pressing the 'Request Info' button in the top right handside corner.

Flow Cytometry Kits

Bio-Techne

R&D Flow Cytometry Kit - Flow cytometry kits designed for analysis of cell populations use up to four fluorochrome-conjugated antibodies. Flow cytometry facilitates quantitative, multiparameter analysis of cell populations based on the expression of cell surface and/or intracellular molecules. R&D Systems offers a wide range of biotin-, fluorescein-, phycoerythrin-, or allophycocyanin-conjugated monoclonal antibodies specifically designed to monitor the presence of cytokines and cytokine receptors as well as Fluorokine® kits; labeled cytokines that can be used to monitor the presence of cytokine receptors. Features of the Flow Cytometry Kit:• Simultaneous detection multiple different targets • Rapid & efficient • Single step staining R&D Fluorokine Receptor Detection Kits employ recombinant biotinylated ligands to quantitatively determine cytokine receptor density using flow cytometry. These simple kits allow the user to estimate the percentage of cells within a population which express the receptor of interest. In addition, cell surface receptor density can be assessed. Flow Cytometry Kit Contents (Multi-Color):• Fluorochrome conjugated antibodies • Isotype controls • All necessary buffers Flow Cytometry Kit Contents (Fluorokine Receptor): • Biotinylated recombinant, species specific ligand • Avidin conjugated with fluorescein (at an f:p ratio of 5:1) • Negative control reagents • Blocking antibody • Wash buffer concentrate

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Human/Mouse Embryonic Stem Cell 4-Color Flow Cytometry Kit