Bio-Rad introduces three StarBright Dyes for flow cytometry

The StarBright Violet 570, StarBright Violet 670, and StarBright Violet 710 Dyes offer precise excitation and emission spectra for improved brightness and resolution

7 Jun 2021
Tom Casburn
Associate Editor

Bio-Rad Laboratories, Inc., a global leader of life science research and clinical diagnostic products, has introduced three StarBright Dyes designed for flow cytometry applications.

StarBright Violet 570, 670, and 710 Dyes offer improved brightness and precise excitation and emission spectra, making them suitable for use in multicolor flow cytometry panels. Bio-Rad’s line of StarBright Dyes offers researchers a range of fluorescent nanoparticles conjugated to highly validated flow antibodies.

“The new StarBright Dyes offer improved brightness when compared to other available dyes at similar wavelengths, and they may be used with a wide range of flow cytometers,” said Mike Blundell, Ph.D., Bio-Rad Product Manager, Flow Cytometry, Life Science Group. “The unique profile of the dyes offers enhanced resolution of rare populations and low-density antigens as well as consistent and reproducible staining.”

Key benefits of StarBright Dyes

  • Compatible with new and existing protocols and suitable for use in multiplex panels
  • Resistant to photobleaching and highly stable with minimal lot-to-lot variation
  • Offer reliable Förster resonance energy transfer (FRET) for reproducible data
  • Work with common staining buffers, including special polymer dye staining buffers, for easy integration into multicolor panels
  • Ensure no loss of signal in fixation
  • Offer narrow excitation and emission profiles for improved resolution in new and existing multicolor flow cytometry experiments

The StarBright Dyes are compatible with Bio-Rad’s ZE5 Cell Analyzer, S3e Cell Sorter, and other conventional and spectral flow cytometers.

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StarBright Violet 515 (SBV515) Dye

Bio-Rad

Excitable by the violet 405 nm laser and emitting at 515 nm, StarBright Violet 515 (SBV515) Dye is a perfect replacement for fluorophores that have emission maxima between 500 and 550 nm, such as Pacific Orange, Krome Orange, vioGreen, and Brilliant Violet 510, which can all be detected with a 525/50 filter (or similar) in your flow cytometry experiments. Minimal excitation by other lasers and a narrow emission profile make StarBright Violet 515 the perfect dye for inclusion in multicolor panels. StarBright Violet 515 Dye is also compatible with spectral flow cytometry and has a unique profile allowing incorporation into new and existing flow cytometry panels.

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ZE5 Cell Analyzer

Bio-Rad

The ZE5 Cell Analyzer is the only flow cytometer with the ideal combination of production throughput, smart sample-handling and superior speed and sensitivity to meet your research needs.

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Bio-Rad introduces three StarBright Dyes for flow cytometry