Single-Cell Analysis of Adult Neurons Using ddSEQ for Characterization of Neuronal Subtype

The ddSEQ Single-Cell Isolator is part of the Illumina® Bio-Rad® Single-Cell Sequencing Solution, a platform that brings Bio-Rad's Droplet Digital™ technology and Illumina's market-leading sequencing expertise to single-cell gene expression studies. The ddSEQ Single-Cell Isolator works with the Illumina® Bio-Rad® SureCell™ WTA 3' Library Prep Kit for the ddSEQ™ System. This technology utilizes disposable microfluidic cartridges to coencapsulate single cells and barcodes into subnanoliter droplets, where cell lysis and barcoding occur. RNA-Seq libraries are subsequently prepared and sequenced. Analysis is conducted via BaseSpace. Features and Benefits Process hundreds to tens of thousands of cells per day Sensitive and unbiased characterization of transcriptional signatures Scalable solution to accommodate a wide range of experimental designs Agnostic to mammalian cell size, enabling unbiased profiling of diverse cell populations End-to-end single-cell sequencing solution with simple, powerful data analysis Applications and Uses Assess cell-to-cell heterogeneity and identify new sub-populations Map cell trajectories Dissect transcriptional mechanics Infer gene regulatory networks Neurology research — normal brain function, neurological disease and psychological disorders Immunology research — B Cell and T Cell subunit functional analyses Cancer research — studies of cellular expansion of clonal cells in tumors and mutations that lead to differences in gene expression and drug response Stem cell research — studies to better understand development and differentiation, including reverse engineering tissues Included Components ddSEQ Test Cartridges ddSEQ Cartridge Holder ddSEQ Single-Cell Isolator Power supply and power cord User manual and product documentation