Expert Insight: The future of CIEF-mass spec techniques for protein characterization

Watch this webinar to find out how directly coupling CIEF with mass spectrometry can benefit biopharmaceutical applications

04 Oct 2019

Usually, capillary isoelectric focusing (CIEF) methods rely only on optical detection due to the incompatibility of common CIEF reagents with ESI-MS detection (e.g. methylcellulose, ampholytes). Recent developments show promising results of the CIEF separation of intact mAb charge variants and coupling CIEF directly to mass spectrometry (MS).

Prof. Dr. Christian Neusüß (Aalen University), Dr. Carsten Sonksen (Novo Nordisk).


In this webinar, Prof. Dr. Christian Neusüß (Aalen University) and Dr. Carsten Sonksen (Novo Nordisk) discuss the results of their work on online iCIEF-MS of intact monoclonal antibody samples and analysis of fractions collected from iCIEF separated protein. This is the first online coupling of iCIEF to MS providing direct identification of each intact mAb charge variant. This method even provides unattended repeated fractionation of such variants for further offline characterizations like peptide mapping.


Learn about:

  • Routine protein isoform characterization by MS and peptide mapping, using either direct MS coupling or fractionation
  • Making routine unattended CIEF fractionation possible
  • Whole solution charge-based protein separation and characterization using CIEF

Attend the live webinar on Thursday, October 10, at:

  • 16:00 GMT
  • 11:00 EST
  • 08:00 PST
  • 17:00 CET

Who should attend

  • Scientists working in assay development or biopharmaceutical QC labs, including CROs and CMOs
  • Anyone who works with CIEF, IEX, Gel-IEF, or anything with iso electrical focusing

All webinar participants can request a certificate of attendance, and a learning outcomes summary document for continuing education purposes

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