Expert Insight: How to choose the best LC autosampler design for your application needs – on demand webinar

Experts Dr. Paula Hong and Jennifer Simeone review how each autosampler aliquots and delivers the sample and discuss their differences

18 Dec 2019


Jennifer Simeone and Paula Hong, PhD
Jennifer Simeone, Principal Scientist (left), and  Dr. Paula Hong, Principal Consulting Scientist, Waters 

A liquid chromatography (LC) system is often chosen based on availability, history or personal preference. Many methods are not significantly impacted by the autosampler differences between the systems, but some workflows or applications can benefit from two different types of injectors: the flow-through-needle or the fixed-loop design.

In a free webinar now available on demand, experts in the field, Dr. Paula Hong and Jennifer Simeone from Waters Corporation, discuss the similarities and differences between the ACQUITY UPLC PLUS systems across multiple areas, including design features — such as the manner of needle washing and injection modes — strategies to transfer across autosamplers, as well as workflows that have specific requirements for the autosampler, such as a high-throughput application or one with problematic carryover.

Watch the webinar now and find highlights from the Q&A discussion at the end of the live event below:

Q: What are the best strategies to address carryover with each autosampler?

It will vary for each type of autosamplers. For the flow-through-needle (FTN), there is only a single injection mode, and the most effective tool to adjust for reducing carryover is the needle-wash composition. For fixed loop autosamplers, there are different strategies you can use. For example, the injection mode will impact your carryover, specifically whether you use a full loop, partial loop, or partial loop with needle overfilled. TIn addition, as with the FTN the washes can also impact carryover.

In addition, there's another characteristic that we didn't specifically discuss that applies more to the fixed loop; a valve switch option. By switching the injection valve when the gradient washing step occurs, the carryover that originates from the injection valve can be reduced in FL autosampler.

Q: Is there a difference in cycle time between the flow-through-needle and the fixed loop autosampler?

There can be a difference in cycle time between the two autosamplers. However, the biggest thing that will impact cycle time is the method and washes. On the FTN, the wash is programmed in seconds - it's the default is often six seconds post-injection. Whereas on the fixed loop, two washes are performed because you have your weak needle wash and your strong needle wash. It will take longer on the fixed loop because you're doing two washes.

However, if you're going for the ultimate in speed and cycle time and you are not concerned about carryover, you could choose not to do any wash after injection. They are the fixed loop that would be slightly faster than the flow-through-needle - but this may be a matter of a few seconds.

Learn more about autosampler designs in LC systems: Watch this webinar on demand >>

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