Editorial Article: How Non-Targeted MS Metabolite Profiling can Improve the Taste of Your Beer

Studying proteomics and metabolomics with TOF mass spectrometers from Waters

28 Jul 2015

Dr Jessica Prenni, of Colorado State University

Dr Jessica Prenni, Director of Proteomics and Metabolomics Facility at Colorado State University and a Waters Center of Innovation Program honoree, uses TOF mass spectrometers in her work on protein and metabolite analysis. The use of non-targeting metabolite identification has transformed agricultural breeding approaches, offering a method to predict downstream characteristics from the presence of certain metabolites.

No-one had access to this kind of information beforehand
Dr. Jessica Prenni

Colorado State University

Predicting the malting process

In the study, Dr. Prenni and her team took a large population of breeding barley lines and studied "the influence of varying different genetics, as well as the breeding environment, using duplicate lines grown in different environments", she told us. Through non-targeted metabolite profiling, Dr. Prenni "correlated the metabolites from the barley to its downstream malting quality characteristics", allowing the creation of a model that enabled her and her team to predict "a certain malting quality trait" if a particular metabolite or set of metabolites were present in the barley. Significantly, the metabolite markers were reflective of both genetic and environmental influences on the malting quality traits.

Understandably, the agricultural industry was very excited about this discovery. "No-one had access to this kind of information beforehand," explained Dr. Prenni, but this technique can be applied “to a very broad range of biological questions".  "Our approach is from an analytical chemistry point of view," she went on, "so we don't have a specific biological focus area." This gives her core facility at Colorado State University high flexibility.

Mass spectrometry and non-targeted screening

Mass spectrometry has become increasingly important to Dr. Prenni's facility in recent years. "We primarily use the Waters TOF mass-spectrometers for our non-targeted screening applications,” revealed Dr. Prenni, "and the data independent acquisition mode allows us to collect the MS and MS/MS on a very fast timescale" – crucial for non-targeted screening. Dr. Prenni also explained how Waters' MSE technology allows her team to collect "all the MS/MS data we need" for complete profiling, as it records data without discrimination or pre-selection, allowing completely catalogued samples that can be re-examined at any time.

Waters Centers of Innovation

The Waters Centers of Innovation Program recognizes analytical scientists facilitating breakthroughs in health and life science research, food safety, environmental protection, sports medicine and many other areas.

The future of chromatography

 Dr. Prenni is already looking to the future. In terms of chromatography, she says, the future is "microflow technology, specifically the iKey device from Waters. The robustness of the system, the sensitivity gains over analytical scale chromatography and in the significant ease of use are unmatched”, she explained, “which is especially important when going from nanoflow applications to microflow applications." However, Dr Prenni really sees the future heading towards "incorporation of ion mobility measurements with the MSe data", making this identification process even stronger.

Watch our talk with Dr Prenni and discover what else you might have missed at ASMS 2015 here