In recent years, gene editing and CRISPR/Cas9 (CRISPR-associated protein-9 nuclease) technology has changed the way we do science. We can now target genome editing in highly specific ways and generate isogenic strains that differ by just a single nucleotide change. In this editorial feature, we bring you the latest advances in the field, including upcoming events, and the new products and workflows making gene editing easier and cheaper than ever before.
1. Reduced Sequencing Costs Through Effective Miniaturization of Library Preparation
While the cost of sequencing per nucleotide has declined rapidly over the past ten years, preparation of samples for next-generation sequencing remains a costly component of the process.
In this application note, Labcyte detail a method to miniaturize reaction volumes utilized in the Illumina Nextera XT DNA library kit, thereby reducing the overall cost per reaction while maintaining equivalent data to the standard protocol. The Labcyte Echo® 525 Liquid Handler enhanced miniaturization of library preparation is effective across samples from a range of organisms, providing consistent and high-quality results for whole genome sequencing, while maintaining sample diversity, ratios, and identification within microbiome and metagenomic applications.
2. Affordable, High Quality, High Yield, and Ready to Use DNA Purification
If you need an affordable, easy-to-use DNA kits for routine molecular biology applications, then Porvair Sciences’ new Chromatrap® DNA kits could meet your research needs. Leveraging proprietary buffer chemistry and unique spin column design, Chromatrap® DNA kits are all free from salts, contaminants and inhibitors, providing you with the high recovery of top-quality DNA perfect for downstream applications.
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3. Efficient CRISPR gene editing workflows
In this paper, Bio-Rad Laboratories presents a versatile and efficient CRISPR gene editing workflow to guide you through the main stages of the gene editing process, applicable to a wide range of gene editing experiments. This paper presents the best Bio-Rad products for optimized gene editing at each stage of the four-step process:
4. Improve Your CRISPR/Cas9 Results
The development of the revolutionary CRISPR/Cas9 genome editing tool has enabled scientists around the world to make more accurate edits quickly and more cheaply than previously thought possible. But to enable the highest accuracy requires the right tools, and as workflows are scaled up, manual sample preparation can prove inefficient, time-consuming, laborious and prone to human error.
In this article, we introduce the Gilson PlateMaster® automated pipette, used to streamline CRISPR/Cas9 studies at scale and help scientists maximize the levels of reproducibility in their workflows.
5. The CRISPR Revolution: Changing Life event
This conference from The Royal Society and supported by AstraZeneca on March 7, 2018, will bring together experts from across government, academia and industry, to discuss current gene editing technologies and utilizing the CRISPR/Cas9 system to help understand:
Droplet Digital PCR (ddPCR) enables rapid quantification of genome editing events. Design and order ddPCR assays to detect non-homologous end joining (NHEJ) and homology directed repair (HDR) edits for any target of interest on Bio-Rad’s easy-to-use Digital Assay Site.
7. The Future of Gene Editing
Recent developments in CRISPR technology have reduced the price, time and complexity of genetic engineering, and it’s starting to make a real difference in how we approach this area of science. We’re already starting to cure diseases, with hundreds of clinical trials investigating gene therapy in adults as treatment for genetic conditions, some types of cancer, diabetes, heart disease and HIV/AIDS.
But what does the future really hold for this technology. In this special feature, SelectScience takes a look at what we could expect from the genetic engineering in the future.
|Explore The Future|
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