VolitionRx to use Innova Biosciences’ Biotin Technology in its Epigenetic Research Kits

22 Jul 2013

VolitionRx Limited, a life sciences company focused on the development of blood-based diagnostic tests, has signed a non-exclusive in-licensing agreement with Innova Biosciences, a specialist provider of bioconjugation systems, based in Cambridge, UK. Under the agreement, Innova Biosciences will supply its Lightning-Link® Biotin technology for Volition’s NuQ® epigenetic profiling research kits. The addition of the Lightning-Link technology will help Volition to improve the accuracy of its test, whilst speeding up production of the research kits.

Volition will use Lightning-Link® for biotinylation of antibodies employed in its range of NuQ® ELISA kits available to researchers working in the fields of epigenetics and nucleosome research. Volition is also employing the Lightning-Link technology for development of Nucleosomics®-based clinical tests, including its first blood-based diagnostic test for colorectal cancer. The high yield and enhanced specificity offered by the Lightning-Link® system coupled with simplicity of use provide a significant advantage in the manufacture of Volition’s research and clinical assays.

Cancer diagnosis often requires a series of time consuming tests, with a diagnosis sometimes being made days after specimen removal. This in-licensing deal will assist the company in their development and manufacture of novel, easy to use diagnostic kits.

Dr Jake Micallef, CSO of VolitionRx, says: “We’ve been really impressed with Innova’s products. Their biotin kit is simple to use and proved more durable than many competitors’ kits, and we’ve seen significant improvements in reproducibility since we started to use them regularly. We’re looking forward to continuing to work with the Lightning-Link technology.”

Dr Andy Lane, Executive Director at Innova Biosciences, said, "This deal is another perfect example of how Lightning-Link® is increasingly being used by innovative diagnostic companies like VolitionRx to both maximise R&D output and to simplify the manufacturing process.”

Lightning-Link™ Strepavidin kit

Innova Biosciences Ltd.

Say goodbye to secondary antibodies – Go direct using the World’s Easiest DIY Antibody Labeling Kit - Lightning-Link™! • Eliminates need for secondary antibodies • Reduces the number of wash steps • Ideal for multi-protein analysis – with over 40 available labels • Very easy to use • 100% antibody recovery Lightning-Link™ kits The DIY Lightning-Link™ kit allows you to label an antibody with minimal hands-on time - less than 30 seconds. Simply add your antibody solution to a tube of Lightning-Link™ mix and the label becomes covalently attached to your antibody in a gentle controlled process. Lightning-Link is the easiest and quickest method available for antibody conjugation.Go ‘direct’ Direct antibodies labelling greatly simplifies immunodetection techniques. Without the problems of crossover and/or non-specific binding from secondary reagents it is far easier to obtain high quality, pioneering results in techniques such as flow cytometry, ELISA, Western blotting and immunohistochemistry. A reduction in the number of incubation and wash steps also saves you time and money. The antibody conjugate: Streptavidin is a 53kDa protein purified from Streptomyces avidinii, which has widespread applications in biotechnology due to its very high affinity to the vitamin biotin. Streptavidin is a tetrameric molecule, composed of 4 13kDa monomers, each of which can bind a molecule of biotin.

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Innova Biosciences - Conjugation Services

Innova Biosciences Ltd.

Innova Biosciences – Antibody Conjugation Service Bio-conjugates can be challenging to optimise and time consuming to produce: 1. Too much conjugation may result in the target protein becoming inactive and insoluble. 2. Too little conjugation can result in weak signals. 3. Our competitors require milligrams of your precious material and have a 2 week process time. Fast & FAb™ Antibody Conjugation Service – Innova Biosciences take the hassle, time and effort out of making antibody conjugates. Using state of the art conjugation chemistry, Innova Biosciences have the ability to label microgram to milligram amounts of your antibody within 48 hours. Conjugate optimization (Micro-optimizer™) - Innova Biosciences uses its proprietary technologies to enhance the performance of conjugates. By creating an array of trial conjugates (10 formulations per label) at low microgram scale (10µg per formulation) combined with selection in the assay of interest, the best possible conjugate can be attained. PerfectSCALE™ - All conjugates produced using our micro-optimization™ technology can be scaled over many orders of magnitude without the need for extensive re-development of the process. The inherent simplicity of Innova’s technology means that exceptionally high yields are obtained, with minimal batch-to-batch variation, ensuring consistent performance.

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