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Thermo Fisher Scientific Offers Improved Crosslinking Immunoprecipitation Kit

7 Apr 2009
Veronica Austin
Marketing / Sales

Product news

Thermo Fisher Scientific, the world leader in serving science, introduces the Thermo Scientific Pierce Crosslink Immunoprecipitation (IP) Kit, which extends the functionality of Protein A/G-based IP reactions by adding crosslinking chemistry to the procedure. The new kit now features an improved protocol and buffers that result in high yield of functional antigens using less antibodies (2-10 µg). The irreversible attachment of the antibody and mild elution procedures not only minimizes antibody contamination in the eluted sample but it also allows the resin to be reused multiple times.

The Pierce® Crosslink IP Kit offers convenient sample handling using spin columns that eliminate resin loss and enable more efficient separation. The procedure can be easily scaled up and works with a wide variety of antibody subclasses and species.

The Pierce Crosslink IP Kit supplies sufficient reagents to perform 50 reactions using 10 µl of immobilized antibody support.

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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Thermo Fisher Scientific Offers Improved Crosslinking Immunoprecipitation Kit