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Sign Up for our Free Webinar: Solving the Aggregation Problem of Cells in Suspension Culture

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Register your place for this exciting event and Q&A session

17 Aug 2015
Sarah Thomas
Associate Editor

Expert insights

08:00 PDT / 11:00 EDT / 16:00 BST / 17:00 CEST, September 1, 2015

Discover a new method for developing aggregation-free cells in suspension culture in our upcoming free webinar, Solving the Aggregation Problem of Cells in Suspension Culture. Don’t miss out – register your place today!

Mammalian cells have become the dominant system used to develop biopharmaceuticals, due to their ability to properly fold and assemble proteins and add post-translational modifications such as glycosylation. Growing them in suspension culture or adapting them to suspension allows for higher yields required for downstream processing. However, some cells, when grown in suspension, start to clump together and there are many solutions available commercially to combat this problem.

In this webinar, Stacey Willard, a Senior Scientist at Eppendorf, will discuss an efficient new method that was used to develop aggregation-free HEK-293 cells in suspension from attachment culture.

By watching this webinar, you will learn:

  • A basic understanding of culturing cells in suspension, including the advantages and disadvantages.
  • A novel approach to solving cellular aggregation that occurs when culturing certain cells in suspension.
  • Tips on how to adapt this method to increase the overall efficiency of your cell line development process.

The Q&A session of the webinar will also provide the opportunity for you to ask any questions you have about the technique.

Register now to secure your place for this exciting online event. If you can't make the live event, don’t worry – we will send all registrants a link to the on-demand version after the event.

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Bioprocessing / FermentationBioprocessing is the use of biological materials to perform commercial, scientific or medical research processes. Biological materials used include cells, enzymes and organisms. Usually bioprocessing requires a batch or continuous bioreactor such as a fermentor or cell culture system. The advantages of using a reactor include high productivity, easy configuration, adjustable values and automation.Protein ExpressionProtein expression is the utilization of cell machinery for the synthesis of proteins and has become a critical tool in biotherapeutic, genomic, and proteomic research. Produce recombinant proteins with expression vectors in combination with a host cell suitable for high-level protein expression. For production of toxic proteins, consider cell-free expression vectors. Create and monitor post-translational modifications with protein modification kits. Find the best protein expression products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.AggregationBiopharmaceuticalsBiopharmaceuticals are proteins and other compounds (such as nucleic acids) produced by living organisms that have uses as therapeutics or for in vivo diagnostics. The most well known example of a biopharmaceutical product, and the first to be approved for therapeutic use, was recombinant human insulin.Glycosylation

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Sign Up for our Free Webinar: Solving the Aggregation Problem of Cells in Suspension Culture