Sick of wasting time, effort and money on secondary reagents?
28 Jun 2009The Lightning-Link™ kit allows antibody conjugates to be set up in seconds, simply by adding a solution of the protein to be labeled to a proprietary lyophilised mixture containing Alkaline Phosphatase (AP). By circumventing the desalting or dialysis steps that commonly interrupt traditional protein conjugation procedures, Lightning-Link™ technology can be used to label small quantities of protein with 100% recovery and without excessive dilution of the conjugate!
Upon dissolution of Lightning-Link™ mixture with a solution of the antibody (or other biomolecule to be labeled) proprietary chemicals in the mixture become activated. This results in the directional coupling of the antibody to the enzyme label in a gentle and controlled process at near-neutral pH. The hands-on time for the entire procedure is typically 20-30 seconds. Lightning-Link™ makes it possible to label primary antibodies and other proteins with ease, and eliminates the need for secondary reagents in immunoassay procedures such as Western blotting, ELISA and immunocytochemistry.
Alkaline phosphatase (AP) is a hydrolyase enzyme that is frequently conjugated to antibodies for use in immunoassays. AP has a lower catalytic rate than HRP, but has greater stability and maintains linear reaction kinetics for much longer. PNPP is a popular colorimetric substrate for AP; the product of the reaction, p-nitrophenol, can be read at 405nM. Fluorogenic substrates (e.g. 4-methylumbelliferyl phosphate; MUP) may also be employed.
Please note as well as AP, we currently have over 40 available labels including enzymes, fluorescent proteins and dyes that span a variety of applications (WB, IH, FACS, ELISA, and FRET), colours, and emission ranges
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Lightning-Link™ Atto 610 kit
Innova Biosciences Ltd.Say goodbye to secondary antibodies – Go direct using the World’s Easiest DIY Antibody Labeling Kit - Lightning-Link™! • Eliminates need for secondary antibodies • Reduces the number of wash steps • Ideal for multi-protein analysis – with over 40 available labels • Very easy to use • 100% antibody recovery Lightning-Link™ kits The DIY Lightning-Link™ kit allows you to label an antibody with minimal hands-on time - less than 30 seconds. Simply add your antibody solution to a tube of Lightning-Link™ mix and the label becomes covalently attached to your antibody in a gentle controlled process. Lightning-Link is the easiest and quickest method available for antibody conjugation.Go ‘direct’ Direct antibodies labelling greatly simplifies immunodetection techniques. Without the problems of crossover and/or non-specific binding from secondary reagents it is far easier to obtain high quality, pioneering results in techniques such as flow cytometry, ELISA, Western blotting and immunohistochemistry. A reduction in the number of incubation and wash steps also saves you time and money. The antibody conjugate: Atto610 is part of the new generation of fluorescent labels for the red spectral region. The dye presents high fluorescence emission at 640nm when excited at 575nm (extinction coefficient 1.5 x105 cm-1M-1) and high quantum yield. Atto610 can be used as a substitute dye for DY-610, BODIPY 630/650 and LightCycler Red 640 and Carboxynapthofluorescein.