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Rockland Immunochemicals, Inc. offers over 2800 target-specific antibodies to validate knockdown protein expression

Rockland Immunochemicals
3 May 2007

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RNA Interference (RNAi) is one of the most exciting discoveries of the past decade in functional genomics and proteomics. Analysis of siRNA transfected cells require high titer, high affinity antibodies.

Rockland Immunochemicals, Inc. is currently offering over 2800 target-specific antibodies to validate knockdown protein expression in a broad variety of mammalian cell types. Our antibodies designed to a large selection of proteins including tumor suppressors, kinases, transcription regulators, cell cycle proteins, membrane receptors, kinases, signaling intermediates and proteins involved in lymphocyte signaling. To access gene silencing, we offer antibodies for Western blot detection and/or immunofluorescent cell staining as well as gene-specific RT-PCR primers that can be used to detect mRNA.

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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.RNA InterferenceRNA interference (RNAi) uses siRNA or miRNA for transcriptional silencing, gene knockdown and regulation of gene expression. RNAi requires chemical synthesis, introduction of DNA vectors into cells, an assay of RNAi effects and RNAi quantification or analysis. Consider target sequence selection, reagent preparation, controls, high specificity and effectiveness and low non-specific gene knockdown.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :

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Rockland Immunochemicals, Inc. offers over 2800 target-specific antibodies to validate knockdown protein expression