Roche Diagnostics France and PrimaDiag Join Together to Propose an Automated Solution for the Capture of Exomes for Sequencing

20 Apr 2015
Kathryn Rix
Administrator / Office Personnel

Roche Diagnostics France and PrimaDiag announced they have concluded a joint promotion agreement whose aim is to propose an automation solution for Roche’s capture of exomes sequencing product range NimbleGen with PrimaDiag’s automation platform ACSIA NGSCaptureEdition.

This agreement is the result of the combined expertises of the two companies, PrimaDiag offers, on one hand, automation of protocole solutions in the field of molecular biology and on the other hand, Roche Diagnostics France furnishes its’ method for liquid phase sequence capturing allowing for the enrichment of genomic regions of interest.

The automation solution on the ACSIA NGSCapture Edition platform is possible for the capture of exomes for all the sequencing products in the NimbleGen SeqCap Enrichment System (EZ, Epi, RNA) product range and for the preparation of samples for exomes libraries . The automation allows the capture from 1 to 48 samples and increases productivity while decreasing the quantity of reagents and consumables that are used during the different steps of a protocol. The automation of the capture of exomes for sequencing reduces the risks of error during the manipulation of the tubes of reagents while increasing the reproducibility of inter-samples. The flow is thus increased and the costs of sequencing are decreased.

“Working with Roche Diagnostics France on the automation of the NimbleGen SeqCap kits is a unique opportunity for PrimaDiag. The quality of Roche’s NimbleGen products is a natural fit with PrimaDiag’s desire to show the efficiency of its ACSIA products solutions as well as the expertise of their respective teams.

The result of this partnership is the development of automation methods which respect and optimize the intrinsic qualities of the NimbleGen kits.

Through this partnership, Roche Diagnostics France and PrimaDiag put within the reach of all the laboratories an automated solution of high quality for the capture of exomes for sequencing” declared Grégory Meunier, General Manager, PrimaDiag.

“By combining our Roche NimbleGen capture of exomes for sequencing solutions with PrimaDiag’s line of ASCIA automated platforms, we allow researchers to respond to the increased number of samples to be analyzed and also to the increased of the number of genes to be studied and to be detected by sequencing”. Carole Donne-Goussé, Product Manager, Roche Sequencing.

Thanks to this collaboration, this complete and successful solution will allow customers to benefit from a flexible and simplified workflow.

ACSIA NGSCapture Edition

PrimaDiag

ACSIA NGSCapture Edition, the solution to automate the steps of library preparation and, more specifically, of target enrichment. ACSIA NGSCapture Edition allows you to perform 48 samples without human intervention or 96 samples with one intervention during the enrichment step. To do so, it operates from just after the hybridization up through the final PCR amplification. It is in this last step at the end of the process that a sufficient quantity of interesting fragments is obtained. Moreover, with our software and its innovative SMT technology, it ensures lower process costs by reducing the quantity of tips used during your manipulations. ACSIA NGSCapture Edition minimizes the use of your reagents and consumables during your process and prevents waste. It also ensures minimal sample loss during treatment. At the end of your processes ACSIA NGSCapture Edition offers a greater diversity of newly created exome libraries and highly reduced process costs.To perform the capture step, ACSIA NGSCapture Edition is available with 16 SBS positions integrating our patented Magnetic Work Unit, our heatingShaker 3000 and a heatingPlate. This configuration is provided with a 250µl 1-channel pipetting head and a 250µl 8-channel pipetting head. Its PrimaController®II software offers both a realistic and interactive view as well as real-time assistance to increase your efficiency by letting you focus on your own tasks.Features: Magnetic Work Unit based on our patented APIM technology (Attraction Point by Induced Magnetization) Pipetting heads are interchangeable within seconds High efficiency automated platform managed by innovative, smart software Easy and User-Friendly programming Pre-programmed protocols for enrichment Add-in our protective enclosure for better safety during your processes One of the market’s best quality/price values

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ACSIA NGSLibPrep Edition

PrimaDiag

ACSIA NGSLibPrep Edition, the solution to automate the libraries preparation for next-generation sequencing. In view of increasing your productivity, avoiding contamination and improving your work efficiency, PrimaDiag created ACSIA NGSLibPrep Edition. It uses our software PrimaControlleur®II and has been designed to allow you to effectively perform your libraries preparation.ACSIA NGSLibPrep Edition allows you to perform 96 samples without human intervention libraries. To do so, it operates from just after the fragmentation up through the final PCR amplification. It is in this last step at the end of the process that a sufficient quantity of interesting fragments is obtained. Moreover, with our software and its innovative SMT technology, it ensures lower process costs by reducing the quantity of tips used during your manipulations. ACSIA NGSLibPrep Edition minimizes the use of your reagents and consumables during your processes and prevents waste. It also ensures minimal sample loss during treatment. At the end of your processes the yield with ACSIA NGSLibPrep Edition is increased by 20% over manual treatment and process costs are and highly reduced.To perform the libraries preparation, ACSIA NGSLibPrep Edition is available with 16 SBS positions integrating our patented Magnetic Work Unit and our heatingShaker 3000. This configuration is provided with a 250µL 1-channel pipetting head and a 250µL 8-channel pipetting head. Its’ PrimaController®II software offers both a realistic and interactive view as well as real-time assistance to increase your efficiency by letting you focus on your own tasks.Features: Magnetic Work Unit based on our patented APIM technology (Attraction Point by Induced Magnetization) Pipetting heads are interchangeable within seconds High efficiency automated platform managed by innovative, smart software Easy and User-Friendly programming Pre-programmed protocols for exome capture Add-in our protective enclosure for better safety during your processes One of the market's best quality/price values

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