Revolutionary Technological Advancement Allows <i>In-Situ</i> Transfection of Living Tissues

11 Sept 2007

Product news

An in-situ transfection system, which has been developed to provide direct transfection of living plant, animal cells and tissues, is the latest addition to the CLEAVER SCIENTIFIC range of laboratory products. The multiTRANF is the first transfection system to enable not only in-vivo and in-vitro transfection but also in-situ particle bombardment for genetic transformation of living tissues. The MultiTRANF provides users with features not available on other systems, for example ultra-fine 1 bar increment pressure control combined with low pressure transfection, necessary for keeping in-situ tissues intact.

There are many convenience and time-saving advantages in using in-situ rather than in-vitro or in-vivo transfection to the research industry. Transfection of intact plants and animals such as drosophilia embryos, allows the transfected organism to be studied without further preparations.

The multiTRANF system can be used with either Helium or Nitrogen gas and operates without the need for consumables, so users will benefit not only from minimal preparation time but also reduced initial experimentation costs, as well as lower on-going running costs. Among the important features and benefits of the system are simple and user-friendly microprocessor control using one-touch buttons, easy and accurate shooting pressure adjustment between 1-100 bars, with 1 bar increments.

A 1-150 bars option is also available. Initial set-up on-site is simple and convenient while optimisation is not dependent on special kits. Start-up packs are available and users have the benefit of an extensive library of users and publications for reference.

MultiTRANF is ideal for use with most animal and plant tissues, embryos, yeast, bacteria, microbes and organelles.

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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Gene Expression and Molecular CloningMolecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Revolutionary Technological Advancement Allows <i>In-Situ</i> Transfection of Living Tissues