Promega PowerPlex Fusion Delivers More Information for Challenging Forensic Casework, Databasing and Paternity Cases
24 Sept 2012Promega Corporation has announced the launch of the PowerPlex® Fusion System, a 24-locus multiplex that incorporates common and informative loci used throughout the world. The PowerPlex Fusion System enables increased data sharing and greater discriminatory power than seen in previously available STR systems. Rapid cycling protocols cut thermal cycling time in half compared to current popular systems, enabling laboratories to focus on more value-added activities.
The system works with extracted DNA, including difficult samples with lower amounts of template and those containing inhibitors. Streamlined protocols also are provided for reference, database and paternity testing for a variety of sample types including swabs, FTA® card punches, and non-FTA punches such as S&S 903 and Bode Buccal DNA Collectors™ devices. Less than 90 minute cycling times and reduced sample preparation times significantly decrease the total time needed to process samples-saving many laboratories more than a half day per 96-well plate.
Promega worked closely with many forensic laboratories around the globe to perform rigorous alpha tests. These tests focused on product performance, including reproducibility, sensitivity and inhibitor tolerance. Based on their experience with the system, most testers expressed an interest in purchasing the PowerPlex Fusion System for use in their laboratories.
Users can consistently generate full DNA profiles from as little as 100pg of input DNA, even in the presence of excessive amounts of inhibitors. The PowerPlex Fusion System is designed to work with existing instrumentation and allele-calling software-no upgrades are required-simplifying laboratory validation efforts. The PowerPlex Fusion System is configured so that both casework and databasing sections of a laboratory purchase the same kit, simplifying validation and QC efforts.
Developmental validation efforts are already underway. In addition, Promega is seeking United States NDIS approval for the PowerPlex Fusion System.
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PowerPlex® Fusion System
Promega Corp.The PowerPlex® Fusion System is a 24-locus multiplex for human identification applications including forensic analysis, relationship testing and research use. The PowerPlex® Fusion System provides all of the materials needed for co-amplification and five-color fluorescent detection of 24 loci (23 STR loci and Amelogenin), including the CODIS core loci and the European Standard Set (ESS) loci. With 24 loci, the system offers the most STR loci and highest discrimination from a single reaction and delivers more information in demanding forensic, paternity and relationship testing cases. Utilizing proven STR chemistries on existing instrument platforms and software, the PowerPlex® Fusion System requires no software or instrument upgrades. The PowerPlex® Fusion System works well with extracted DNA samples, including low amounts of template DNA, mixtures and inhibitor-laden samples. The PowerPlex® Fusion System also is compatible with direct amplification, enabling streamlined STR databasing efforts. Amplification can be successfully performed with sample types such as FTA® card punches as well as pretreated swabs, Bode Buccal DNA Collector™ punches or S&S 903 punches. Fast cycling conditions used with PowerPlex® Fusion reduce sample processing time for all samples. PowerPlex® Fusion System Benefits: Achieve the most inter database compatibility and highest discrimination of any autosomal STR kit. Improve laboratory efficiencies with rapid cycling and direct amplification protocols. Obtain a higher success rate with difficult casework samples due to robustness and sensitivity. Simplify validation and QC efforts by using one kit for both casework and databasing sections.