Optimization of mRNA delivery to overcome transfection challenges in hiPSC-derived neurons and microglia

The optimization of mRNA transfection remains a critical challenge, with researchers seeking higher efficiency, improved cell viability, and reproducible delivery across diverse cell types. Understanding how to fine‑tune mRNA dose, formulation, and transfection conditions is essential for advancing disease modelling, cell engineering, and therapeutic development.

This scientific poster shows how systematic optimization strategies enhance mRNA uptake and expression. The results provide actionable insights, quantitative performance data, and practical guidance that can directly support scientists and researchers working with mRNA‑based technologies.