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Novus Biologicals Announces New Antibody for Stem Cell Research

Novus Biologicals, LLC
25 Jan 2011
Sarah Sarah
Marketing / Sales

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Novus Biologicals announces a new rabbit polyclonal anti-Lin28 antibody for stem cell research (catalog # NBP1-49537). This antibody is useful for immunofluorescence, immunohistochemistry and Western blot.

Novus' new Lin28 antibody is useful for immunofluorescence, immunohistochemistry and Western blot. By Western blot, Lin28 antibodies recognize a band at approximately 29kDa. This new Lin28 antibody was validated by Western blot on NTERA-2 cell lysate and 293T cells overexpressing LIN28. This Lin28 antibody cross-reacts with human and mouse. The immunogen to which this antibody was made is a partial recombinant human Lin28 protein [Swiss-Prot# Q9H9Z2] expressed in E. coli.

Lin28 is marker of undifferentiated human embryonic stem cells and has been used to enhance the efficiency of the formation of induced pluripotent stem cells from human fibroblasts. Lin28 acts as a 'translational enhancer,' driving specific mRNAs to polysomes and thus increasing the efficiency of protein synthesis.

As an embryonic stem cell marker, Lin28 is closely related to Sox2, OCT4 and Nanog.

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AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.Western BlottingWestern blotting equipment is used to transfer and identify specific proteins within a sample, reveal protein modifications, as well as give a semi-quantitative estimation of their concentration. Western blotting equipment includes all apparatus necessary to transfer proteins from gel to membrane and subsequent processing steps. Protein transfer can be performed by electroblotting with wet, semi-dry and dry transfer systems onto nitrocellulose and PVDF membranes. Blocking, washing and labeling of membranes follows, involving buffers, blocking reagents, blotting / incubation trays, labeling reagents, immunoblotting assays, antibodies and conjugates. Automated equipment for these steps is available to accelerate your lab workflow. Finally, detection and imaging of proteins can be conducted using gel documentation and imaging systems. Find the best western blotting equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.RNA InterferenceRNA interference (RNAi) uses siRNA or miRNA for transcriptional silencing, gene knockdown and regulation of gene expression. RNAi requires chemical synthesis, introduction of DNA vectors into cells, an assay of RNAi effects and RNAi quantification or analysis. Consider target sequence selection, reagent preparation, controls, high specificity and effectiveness and low non-specific gene knockdown.

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Novus Biologicals Announces New Antibody for Stem Cell Research