SelectScience
Join Free
NewsLife Sciences

Novel reagents advance research into Parkinson's disease and sleep disorder

AMSBIO

AMSBIO reagents drive progress in Parkinson's, hypersomnia, and eco-friendly microbial research

28 Feb 2024
Will Thompson
Editorial Assistant

Product news

amsbioreagentprimage2024
BioPORTER® protein delivery reagent kit enables direct translocation of proteins into living cells

AMSBIO describes how life science research groups are using its BioPORTER®, Detachin™, and SoluLyse™ reagents to make advances in Parkinson’s research, hypersomnia studies and eco-friendly microbial ester production.

Novel reagents empower research by enhancing the efficiency, accuracy, and reproducibility of scientific investigations. A collaborative effort led by Dr. Wenjing Wang at the University of Michigan and Dr. Xiaobo Mao of Johns Hopkins University School of Medicine has made exciting advances in the development of potential therapeutics for Parkinson’s disease.

To determine the relationship between α-syn aggregation and PFFNB2 in mammalian cells, Dr. Wang and Dr. Mao used a BioPORTER protein delivery reagent kit to enable easy transduction of α-syn preformed fibrils to HEK293T cells. BioPORTER uses biocompatible polymers to encapsulate and protect proteins during delivery, ensuring stability and activity, and improving chances for more accurate and reproducible results.

Dr Andrew Jenkins and his team at the University of Saint Joseph are pioneers in repurposing compounds to address the debilitating effects of sleep disorders including hypersomnia. Focusing on eleven compounds previously identified as modulators of GABAA receptor brain activity to alleviate excessive sleepiness, Dr. Jenkins team use Detachin Cell Detachment Solution from AMSBIO to prepare cells for electrophysiological analysis. Cell detachment is a critical step in cell culture experiments. Using Detachin, the University of Saint Joseph researchers were able to reliably produce high cell viability and functionality, reducing the risk of altered cell behavior due to harsh detachment methods.

Microbial production of short-chain esters offers an eco-friendly alternative to chemical synthesis, but low yields remain a challenge. To address this, Dr. Arul M. Varman at Arizona State University and Dr. Ryan Davis from Sandia National Laboratories used SoluLyse Bacterial Protein Extraction Reagent to simplify and accelerate the process of obtaining high-quality protein extracts from E. coli. Using this efficient formulation – the research teams found SoluLyse to be a great asset in their protein research, ensuring efficient cell lysis without compromising protein integrity.

Want the latest science news straight to your inbox? Become a SelectScience member for free today>>

Related products

Request Quote for All Products

Links

AMSBIOCompany website

Tags

ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :Clinical TrialsClinical Trials, an essential part of drug discovery process, assess the safety and effectiveness of a new medication or device in the pharmaceutical industry. Clinical Trials are a phased process (Phase 0, Phase I, Phase II, Phase III and Phase IV) which begins after initial preclinical testing.Compound AnalysisCompound analysis is used to discover specific compounds that could be promising candidates for pharmaceutical use. This potential is identified when compounds have the desired effect, such as interaction with a target protein, during high throughput screening.Drug DeliveryDrug Delivery refers to dosage form, route of administration, formulations, technologies, and systems for transporting a pharmaceutical compound in the body as needed to safely achieve its desired therapeutic effect. Drug delivery is often approached via the biopharmaceutical or small molecule drug's formulation, but it may also involve medical devices or drug-device combination products. Considerations include instrumentation, software and services. Bioprocessing / FermentationBioprocessing is the use of biological materials to perform commercial, scientific or medical research processes. Biological materials used include cells, enzymes and organisms. Usually bioprocessing requires a batch or continuous bioreactor such as a fermentor or cell culture system. The advantages of using a reactor include high productivity, easy configuration, adjustable values and automation.Protein QuantificationThe detection and quantification of proteins in a sample is vital across life sciences, pharmaceutical research and clinical diagnostics, and a variety of equipment is available to scientists to simplify the workflow. Proteins of interest can be easily labeled and detected on light-based detection instruments. Immunoassay kits allow you to identify a specific protein of interest and protein detection beads or antibody microarrays allow you to identify multiple specific proteins at once. Protein interactions and enzyme activity can also be monitored with protein-protein interaction assays. Additionally, the biophysical characterization of proteins is made easy with biokinetic analyzers. Find the best protein detection and quantification products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicrobiologyMicrobiology is the study of microorganisms including protists, prokaryotes, fungi, and, often, viruses. Microorganisms are a useful research tool as genetic vectors and, in immunology, for antibiotic susceptibility testing, cellular biology and genetics. Microorganisms commonly grow readily in incubators with microbial culture media; this can contain chromogenic supplements to differentiate between cell lines. Estimate your culture’s density of microorganisms with colony counters, or screen and select colonies for desirable clones with automated colony pickers. Additionally, equipment is available to monitor environments for the presence of microbes and identify with microbial identification instruments. Find the best microbiology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Reagent DispenserMicrobial DetectionMicrobial detection is crucial for identifying and quantifying bacteria, viruses, fungi, and other microorganisms. This process is essential for food safety, environmental monitoring, clinical diagnostics, and pharmaceutical manufacturing. Explore the best microbial detection tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Parkinsons DiseaseBacterial IdentificationProtein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.

Related articles

Novel reagents advance research into Parkinson's disease and sleep disorder