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New Nano LC System and Columns for Proteomics Research Launched at HUPO 2015

Thermo Fisher Scientific

Designed and preconfigured for straightforward, state-of-the-art separations

28 Sept 2015
Lois Manton-O'Byrne, PhD
Executive Editor

Product news

Proteomics laboratories can benefit from a new nano LC system with dedicated columns as front-end for liquid chromatography mass spectrometry (LC-MS). The new Thermo Scientific EASY-nLC 1200 system with a pressure capacity of 1200 bar is designed to provide effortless separation performance and high-throughput capacity.

The Thermo Scientific EASY-nLC 1200 system and the Thermo Scientific Acclaim PepMap nanoLC columns will make their debut at the Human Proteome Organization’s 14th Annual World Congress, Booth 301 at the Vancouver Convention Center, Vancouver, British Columbia.

The EASY-nLC 1200 was designed for robust and reliable operation at 1200 bar. New maintenance-free ceramic valves decrease system downtime and tool-free nanoViper connections provide fast and reproducible fluidic connections over the complete flow path. For simplified operation and maintenance, new software features enhance performance and usability. As a result, users at various levels of expertise receive an optimized and fully integrated solution designed to consistently generate high quality data.

“The EASY-nLC 1200’s ability to run at higher pressure enabled us to more rapidly load samples, hence dramatically improving sample throughput,” said Thomas Kislinger, Associate Professor at the Princess Margaret Cancer Center, University of Toronto.

The new Acclaim PepMap nanoLC columns are designed to handle the 1200 bar pressure capability of the EASY-nLC 1200. In particular, the new 75 cm format provides high peak capacity intended to increase sensitivity and resolution. This capability increases the number of protein identifications for improved proteome analysis which delivers excellent confidence in LC-MS results.

“In combination with our leading mass spectrometry technologies, the EASY-nLC 1200 pushes the boundaries in proteomics without compromising robustness,” said Remco Swart, director of LC-MS technologies at Thermo Fisher Scientific. “This newest addition to our nano LC-MS solutions marks an important milestone that brings proteomic laboratories to new levels of biological information.”

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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.LC-MSLC-MS (liquid chromatography-mass spectrometry) systems and equipment are used for separation and quantitative analysis of complex mixtures, combining liquid chromatography and mass spectrometry. Quantify proteins, contaminants, pesticides or screen for drug metabolites with a high level of sensitivity. LC-MS systems and equipment include reverse phase, normal phase and specialized columns integrated with various MS detectors such as time-of-flight (TOF), quadrupole, orbitrap or ion trap mass analyzers. LC-MS/MS instruments equipped with a qTOF or triple quadrupole analyzer give greater sensitivity and resolving power to your analysis. Find the best LC-MS equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer. ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein InteractionsProtein interactions are essential for understanding cellular processes, as proteins work together to carry out biological functions. Studying protein interactions can provide insights into disease mechanisms, drug development, and cell signaling pathways. Explore the best tools for protein interaction research in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.LC-MSLiquid Chromatography-Mass Spectrometry (LCMS) is a powerful analytical technique that combines the separation power of liquid chromatography with the detection capabilities of mass spectrometry. It is widely used for qualitative and quantitative analysis of complex mixtures in pharmaceuticals, proteomics, and environmental studies. Browse our peer-reviewed product directory to find the best LCMS systems, compare products, check reviews, and get pricing directly from manufacturers.

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