New England Biolabs Releases the NEBNext® Microbiome DNA Enrichment Kit
3 Jun 2013
New England Biolabs (NEB) announces the release of the NEBNext® Microbiome DNA Enrichment Kit, which uses a novel method to separate microbial DNA from host DNA, thereby reducing the high cost of sequencing microbiome DNA to a more practical level.
Microbiome samples are commonly dominated by host DNA (up to 99%). This complicates the genetic analyses of these samples, particularly total microbiome DNA sequencing studies. Since only a small percentage of sequencing reads pertain to the microbes of interest, obtaining sufficient sequence coverage of the microbiome DNA can become cost-prohibitive or even technically infeasible.
The NEBNext Microbiome DNA Enrichment Kit utilizes the MBD2-Fc protein, which binds to CpG-methylated DNA (including human genomic DNA) with very high specificity. The MDB2-Fc protein is attached to Protein A-coated magnetic beads, enabling quick and easy removal of the contaminating host DNA in about 30 minutes. The microbial DNA-enriched sample is then ready to be processed for multiple downstream applications, including next generation sequencing, qPCR and endpoint PCR.
“Although microbiome whole genome sequencing provides more information than other methods, such as 16S analysis, this method has proven difficult for many samples, due to the presence of contaminating host genomic DNA,” says Fiona Stewart, Product Marketing Manager, Next Generation Sequencing at NEB. “The NEBNext Microbiome DNA Enrichment Kit makes it possible to substantially enrich samples for non-host, microbial DNA, while retaining microbial diversity, thereby improving the quality and cost-effectiveness of downstream analyses.”
Related Products
Request Quote for All Products
NEBNext® Reagents for DNA Library Preparation
New England Biolabs Inc.NEBNext® reagents are a series of highly pure reagents that facilitate library preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. Available in sets, master mixes and modules, these robust reagents undergo stringent quality controls and functional validation, ensuring maximum yield, convenience and value. Advantages of NEBNext Reagents for RNA Library Preparation: Convenient formats – Enzymes, buffers and nucleotides are included, at appropriate concentrations and in appropriate volumes, and are available in set or master mix format. Large and small sizes are available. Functional Validation – Each set is validated together by preparation of a library from an accepted RNA reference sample, that is sequenced using an Illumina GAIIx. Stringent Quality Controls Value Pricing Novel Protocol for small RNA - Higher yield and substantially reduced adaptor dimer formation suitable for methylated and unmethylated small RNAs. Applications of NEBNext Reagents for RNA Library Preparation: mRNA library preparation for next generation sequencing Small RNA library preparation for sequencing with Illumina and SOLiD Expression library construction