Kapa Biosystems Introduces PROBE FORCE- an Amplification-free qPCR Master Mix for Challenging Sample Types
25 Aug 2015Kapa Biosystems announced the introduction of KAPA PROBE FORCE, a highly inhibitor resistant qPCR master mix that removes the need for DNA purification, enabling streamlined sample-to-quantitation in less than an hour.
The master mix contains a high-performance engineered DNA polymerase enzyme specially designed to overcome blood, tissue, and plant PCR inhibitors. With KAPA PROBE FORCE, crude samples can now be analyzed with comparable accuracy, reproducibility, and sensitivity as purified DNA. The product is designed for low- or high-throughput genotyping, as well as gene expression studies.
“KAPA PROBE FORCE is ideally suited for direct qPCR analysis of challenging sample types without the need for purification,” said Charles Joseph, Global Product Manager, qPCR at Kapa Biosystems. “Customers can create streamlined, cost-effective workflows designed to fit any budget, without compromise.”
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KAPA PROBE FORCE qPCR Kits
Kapa Biosystems, Inc.KAPA PROBE FORCE is a highly inhibitor resistant qPCR master mix that removes the need for DNA purification, enabling streamlined sample-to-Cq workflows. The master mix contains a third generation DNA polymerase evolved to overcome blood, tissue, and plant PCR inhibitors. Crude samples can now be analyzed with comparable accuracy, reproducibility, and sensitivity as purified DNA using KAPA PROBE FORCE. Features: Streamline sample-to-Cq workflows in <1 hour. Eliminate the time and cost of sample purification by amplifying directly from crude samples. Analyze a wide range of sample types including whole blood, cells, mouse tails, FFPE, leaf, stem, seed, and soil. Generate accurate and reproducible results. Kits include a third-generation DNA polymerase, evolved for robust target amplification and detection. Enzyme maintains high reaction efficiency in the presence of PCR inhibitors for reliable data generation. Break through high levels of qPCR inhibitors. Achieve greater levels of sensitivity for inhibited blood, tissue, and plant samples. Convert purified DNA assays to crude workflows without observable Cq delays. Multiplex crude samples efficiently. Accelerate genotyping analysis with single reaction allelic discrimination of crude DNA extracts. Maximize data collection from precious samples, increase throughput, and reduce costs. Applications: GMO testing. Mouse transgenics. SNP genotyping. Food/water pathogen detection. Infectious disease research. Cancer research.