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Infinite™ 200 in search of biological insecticides

Tecan
18 Sept 2007

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Scientists at the Microbial Ecology and Host-Pathogen Interaction Laboratory (EMIP), of the INRA Institute in Montpellier, France, have chosen Tecan’s monochromator-based Infinite™ M200 microplate reader to monitor real-time gene expression in insect pathogens. The researchers are studying bacterial virulence genes, aiming to develop a new means of biological control using insect pathogens for the integrated pest management of crops.

Dr Alain Givaudan, head of the Virulence Factors and Bacterial Genomics group, explained: “During real-time monitoring of gene expression, the Infinite M200 simultaneously monitors optical density (bacterial growth) and fluorescence (gene expression) of shaken bacterial cultures in 96-well microplates over 48 hours. The Infinite M200 is very easy to use, and it allows us to obtain plenty of results in a short space of time. Various parameters, like the temperature, agitation or the frequency of measurements, can all be programmed very easily using Tecan’s Magellan™ software.”

Available with a monochromator or a filter system, the Infinite 200 series of instruments offers the choice of fluorescence, luminescence or absorbance detection modes to suit individual needs. It is designed with a modular concept, which allows later upgrades and integration with other systems.

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.MicroplatesMicroplates are multi-well plates used to increase the throughput of biological investigations. The number of wells microplates contain can range from 6 to 3243 wells, with the 96-well format being the most commonly used. Microplates can come tailored for a range of applications including cell culture, PCR, filtration, storage, non-binding surface, protein crystallization, as well as pre-coated, deep well and normal Standard microplates. Additionally, options for microplate colors include clear , black, white or black with clear bottom and white with clear bottom for absorbance microplate reader applications. Find the best microplates for your lab in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Microplate Readers / DetectorsMicroplate readers are used to automate the detection and analysis of labeled or label-free components in microplates during assays or live-cell monitoring. Microplate readers are generally distinguished by their mode of detection. Types include absorbance, luminescence, fluorescence intensity, fluorescence polarization, TRF / FRET and multimode microplate readers. Microplate readers deliver a high throughput of samples by reading multiple wells simultaneously, with the 96-well format the most commonly used. As a result, microplate readers are often used in the drug discovery, bioassays, research and pharmaceutical industries for screening applications. Microplate loading can also be automated, with robotic microplate stackers to increase throughput. Find the best microplate readers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.MicrobiologyMicrobiology is the study of microorganisms including protists, prokaryotes, fungi, and, often, viruses. Microorganisms are a useful research tool as genetic vectors and, in immunology, for antibiotic susceptibility testing, cellular biology and genetics. Microorganisms commonly grow readily in incubators with microbial culture media; this can contain chromogenic supplements to differentiate between cell lines. Estimate your culture’s density of microorganisms with colony counters, or screen and select colonies for desirable clones with automated colony pickers. Additionally, equipment is available to monitor environments for the presence of microbes and identify with microbial identification instruments. Find the best microbiology products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Infinite™ 200 in search of biological insecticides