Improving rheumatoid arthritis assay consistency with recombinant RF IgM

This technical note explores the use of recombinant Rheumatoid Factor (RF) IgM as a reliable alternative to patient-derived materials for use in rheumatoid arthritis (RA) immunoassays. Traditional RF calibrators and controls sourced from human donors are increasingly difficult to obtain and can have significant lot-to-lot variability, creating challenges for assay standardisation and long-term supply.

The recombinant RF IgM described in the note is engineered as a monoclonal human IgM expressed in HEK293 cells, designed to mimic native RF structure while providing consistent performance. The material demonstrates strong commutability with native RF, high activity levels, and reproducible dilutional behaviour across multiple clinical chemistry platforms, including Abbott, Roche, and Beckman systems.

Resource details:

• Resource type: Technical note
• Page count: 3
• Read time: 5 mins