Improve Speed and Sensitivity for your LC/MS Analysis

8 May 2008
Greg Smith
Analyst / Analytical Chemist

Phenomenex presents HPLC options for increasing lab productivity at ASMS 2008. Visit booth #131 to see new products for LC/MS techniques and applications. Poster presentations will introduce Phenomenex research into the analysis of drug compounds in biomatrices and the effect of pH on biological samples in RP LC-ESI+-MS/MS.

Ideal Columns for LC/MS Applications – Booth #131

Jupiter® 300 3µ
Ideal for separating closely eluting proteins, Jupiter 300 3µ columns give better resolution and sharper peaks than other phases. These columns fully resolve post-translational modifications and are ideal for research on proteins such as ctytochrome C and beta-lactoglobulin.

Gemini®-NX
Through a second-generation organo-silica grafting process, Gemini-NX is a rugged column platform that simplifies column selection and enables robust method development for ionizable compounds. One of the most versatile reversed phase columns from Phenomenex, Gemini-NX excels in both MS-volatile buffers and non-volatile buffers, offers high sample loading capacity, and is one of the most durable, pH tolerant columns on the market.

Onyx™ 2.0 mm ID Monolithic
Now you can achieve sample throughput at monolithic speeds! The new 50 mm x 2.0 mm ID format offers solutions for faster sample throughput at low pressures and high efficiencies.

Poster Presentations

• Ideal Sample Preparation and HPLC Column Choice for the Analysis of Drug Compounds in Biomatrices (M, 6/02, 11:15 - 3:30)

• Effect of pH on the Quantification and Ion Suppression of Basic Drugs in Samples of Biological Origin by RP LC-ESI+-MS/MS (M, 6/02, 11:15 - 3:30)

Onyx Monolithic LC Columns

Phenomenex Inc

Acheive Faster HPLCOnyx monolithic silica-based HPLC columns are designed for high-throughput analysis and the direct injection of dirty or viscous samples. Reduce run times with extremely high flow rates (up to 9 mL/min) "Dilute-and-Shoot” dirty biological samples Scalable from capillary to semi-prep column dimensions Onyx columns deliver high efficiency separations and can be coupled together in series to produce the extreme plate counts needed to separate very complex mixtures.Dilute & Shoot Complex mixtures coupled with dirty matrices, such as serum samples encountered in the pharmaceutical/biotechnology industry, are quite difficult and time-consuming to analyze. With Onyx however, you have the option to dilute-and-shoot your sample directly onto the column because it won't clog due to the macroporous system or cause high backpressures. Flow Rate Flexibility Another unique feature is the ability to use flow rate as parameter for optimizing separations. Onyx columns are responsive to changes in flow rate and can be changed mid run to reduce total run time when certain compounds elute much later. This type of flow rate flexibility gives many options for optimization.

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Jupiter LC Columns for Proteins & Peptides

Phenomenex Inc

RP-HPLC for Protein/Peptide Analysis and PurificationJupiter® HPLC columns offer optimized reversed phase solutions for protein and peptide characterization and purification. A wide variety of phases (C18, C12, C5, C4) and dimensions (capillary-preparative)/bulk media are available to support the majority of separation requirements.Jupiter 300 - 300 Å columns designed to purify intact proteinsJuptier Proteo - 90 Å columns engineered for increased peak capacity and resolution of peptide maps as well as peptide separationsExplore Aeris Core-Shell HPLC/UHPLC columns for ultra-high performance protein/peptide analysis and characterization.

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