High-speed spectral acquisition with a single scan; enhanced spectral imaging with the A1Si.

Nikon Instruments’ groundbreaking A1 series of confocal laser scanner systems has now been even further enhanced by the launch of the A1Si spectral detector option which provides fast 32-channel spectral imaging with speeds of up to 16 fps at 512 x 64 pixels and real time spectral unmixing. This cell-friendly system offers the ultimate solution for reducing light exposure during multiprobe studies, capturing full spectral data in just one fast scan.

Fast fluorescence unmixing during image acquisition is possible, with a 512 x 512 pixel, 32-channel image unmixed in less than one second. Coupled with high-speed spectral imaging, a clearly resolved image with no crosstalk between closely overlapping fluorophore emissions can be created in real time.

In addition, a new virtual filter function freely utilises the 32 channels, providing the flexibility to handle any new fluorescence probes. Desired spectral ranges can be selected and the total intensity of each range adjusted individually. Broader band spectral imaging is achieved on the A1Si, which requires only one pass of the laser to capture all the spectral data in the sample.

Designed to take confocal imaging to new levels of speed and resolution, the A1 and A1R are powerful, fully-automated imaging systems capable of capturing high-quality confocal images of cells and molecular events at high speed and enhanced sensitivity; and now allowing high-speed spectral data acquisition with a single scan. The A1 utilises conventional paired galvanometers producing high resolution images (up to 4096 x 4096 pixels); the high specification A1R features an innovative hybrid resonant-non-resonant scanning system capable of capturing images at 30fps (512 x 512 pixels). This facilitates ultra-high-speed imaging with unsurpassed image quality, up to 230fps (512 x 64 pixels). Furthermore, it offers the ability to perform time-lapse imaging and simultaneous PA-GFP or FRAP with reduced photobleaching and phototoxicity.

Nikon’s increased light detection efficiency provides high image quality – allowing brighter images, minimising laser exposure intensity and reducing damage to cells. One scan fluorescence imaging reduces image acquisition time, and protects cells from further light exposure.

Dedicated NIS-Elements C software, based on Nikon’s easy-to-use NIS-Elements, for image capture, processing and analysis for confocal microscope applications is provided as standard, as well as analysis software for FRAP and FRET. Unified integrated control of the entire imaging system (microscope and camera) offers significant benefits for cutting-edge research, such as live cell imaging.

The A1 series, together with Nikon’s Ti inverted microscope, set a new standard for advanced time-lapse studies of rapid cellular interactions to bring biological imaging to life.