GFP expression analysis using the Luna-FL Fluorescence Cell Counter

29 May 2026

This application note demonstrates how the LUNA‑FL™ fluorescence cell counter can be used to measure GFP expression and evaluate transfection efficiency in cultured cells. By detecting fluorescence intensity relative to background controls, the system quantifies the proportion of GFP-positive cells and provides rapid analysis of transfection outcomes. Discover how the workflow combines features of fluorescence microscopy and flow cytometry, enabling fast and accessible assessment of gene expression in cell-based experiments.

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Flow Cytometry / Cell CountingFlow cytometers are used to count, sort and examine multiple characteristics of cells. Other cell analysis equipment includes image cytometers, cell counters, fluorescence-activated cell sorters (FACS), magnetic-activated cell sorters (MACS), and a range of flow cytometry assay kits. Flow cytometers can reveal information on cell viability, cell proliferation, apoptosis and cell cycle progression, as well as identify cell populations and intracellular or cell-surface molecules. Additionally, some flow cytometers, known as FACS, have an additional sorting function after analysis. Cell counters and image cytometers count live and dead cell populations and can also conduct cell proliferation assays. Find the best flow cytometers, cell counters and cell sorters in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.TransfectionTransfection introduces nucleic acids (DNA or RNA) into cells, facilitating gene expression, genome editing, and functional studies. It�s widely used in genetic research, drug discovery, and therapeutic development. Find transfection reagents, kits, and systems in our product directory with reviews and pricing options.GFPCell AnalysisThe analysis of cells allows researchers to understand the factors which contribute to cell health and function. These influencing processes can then be predicted and altered, leading to the development of medication and disease treatments.