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EMD Millipore Launches Simplicon™ RNA Reprogramming Technology for Efficient Generation of Virus-Free iPSCs

Merck
19 Jun 2014
Sarah Thomas
Associate Editor

Product news

EMD Millipore, the Life Science division of Merck KGaA of Darmstadt, Germany, today launched Simplicon™ RNA Reprogramming Technology, which uses synthetic self-replicating RNA to create large numbers of human induced pluripotent stem cells (iPSCs) using a single transfection step. This efficient reprogramming of somatic cells is accomplished without viral intermediates or host genome integration, offering a more defined and safer system for iPSC generation.

"For stem cell researchers using iPSCs to study disease, differentiation and regenerative medicine, there's a real need for a reprogramming method that's as efficient as virus-based techniques yet as safe as non-viral methods," said Christophe Couturier, Head of Bioscience, EMD Millipore. "Simplicon™ technology meets this need, with a single-transfection protocol that's significantly easier than other approaches."

The Simplicon™ RNA Reprogramming Technology uses a single synthetic, polycistronic self-replicating RNA strand engineered to mimic cellular RNA. The RNA strand contains the four reprogramming factors, OCT-4, KLF-4, SOX-2 and GLIS1, and enables the creation of integration- and virus-free iPSCs using only one transfection step. Once the cells are generated, the RNA and reprogramming factors can easily be eliminated from the cell culture medium in a controlled manner.

EMD Millipore is launching the Simplicon™ RNA Reprogramming Technology at the International Society for Stem Cell Research (ISSCR) annual meeting being held June 18-21 in Vancouver, Canada. Attendees can learn more about the technology at the following ISSCR events:

• Innovation Showcase: Virus-free, Efficient Reprogramming Using a Single Transfection of a Synthetic, Polycistronic Self-Replicating RNA
Friday, June 20; 11:30 a.m. - 12:30 p.m. (lunch will be provided)
Vi Chu, Ph.D., Manager II, R&D, Stem Cells/Cell Biology
West Ballroom C/D

• Poster Presentation: Single Transfection of a Synthetic Polycistronic Self-Replicative RNA with Small Molecules Yields High Numbers of Human iPSCs
Thursday, June 19; 6:00 - 7:00 p.m.
Min Lu, R&D Scientist III
Poster T2155

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.Cell Lines Stem Cells and Primary CellsPrimary cell cultures, established cell lines and stem cells are vital for <i>in vitro</i> and <i>ex vivo</i> experimentation. High-quality cells, optimized for your applications, alongside optimized cell substrates, growth medium and supplements, are critical for experimental success. Explore a range of cells suitable for your applications, including isogenic cell lines, competent cells, induced pluripotent stem cell (iPSC)-derived cell lines, fungal/bacterial/mammalian cell lines, stem cells and cancer cell lines. Find the best cells for your research in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.RNA InterferenceRNA interference (RNAi) uses siRNA or miRNA for transcriptional silencing, gene knockdown and regulation of gene expression. RNAi requires chemical synthesis, introduction of DNA vectors into cells, an assay of RNAi effects and RNAi quantification or analysis. Consider target sequence selection, reagent preparation, controls, high specificity and effectiveness and low non-specific gene knockdown.Stem Cell ResearchStem cells have the ability to produce identical daughter cells as well as progeny, which commit and differentiate. Stem cell research has exciting prospects of biomedical applications in cellular disease modeling, pharmaceutical screening, and regenerative medicine. Ipsc

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EMD Millipore Launches Simplicon™ RNA Reprogramming Technology for Efficient Generation of Virus-Free iPSCs