ChromoTek Introduces Chromobody Plasmids for Real-Time Monitoring of Key Intracellular Target Molecules in vivo

ChromoTek GmbH offers nanobody based real time assays to visualize and measure the action of candidate drugs or other substances of interest on important regulatory processes in living cells. Presently, four Chromobody® plasmids are available for analyzing the cell cycle, apoptosis, DNA methylation and cytoskeleton dynamics.

A major advantage of the new Chromobody® plasmids compared to Chromobody® cell lines already on the market is that researchers can now monitor cellular pathways in their own cells or cell lines simply by carrying out a transfection. Unlike conventional end point assays, the Chromobody® plasmids allow to follow the fate of endogeneous target proteins over time like in a movie. As the fluorescently labeled intracellular antibodies expressed from these plasmids do not interfere with the activity of their targets, they are extremely useful for high content screening.

The new product format is equally suitable for academic researchers who want to decipher cellular pathways and to biotech/pharma researchers who seek to enhance their drug discovery and high throughput screening capabilities. “We are glad to be able to offer our customers a new product format that gives them greater flexibility both in basic cell biology research and in drug discovery and validation processes” states Marion Jung, Managing Director of ChromoTek.

Customers who would like to try the Chromobody® technology for their own applications can now choose between three options: they can license a cell line, order assay-ready frozen instant cells via ChromoTek or via CCS (Cell Culture Service, now part of Evotec AG) or purchase a Chromobody® plasmid.