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AVIA Biosystems Automates Protein Stability Studies

1 Oct 2013
Liam McNair
Administrator / Office Personnel

Product news

The new Model 2304 Automated Protein Denaturation System completely automates protein stability determinations using chemical denaturation, and is both the first instrument of its kind and the first instrument to be introduced by AVIA Biosystems of Norton, Mass. Developed to facilitate the formulation of biologics in a safe and stable form, the system uses intrinsic or extrinsic fluorescence to monitor the conformational changes associated with protein unfolding (denaturation) and automatically generates complete protein stability curves.


Up to 96 different formulations, process conditions or individual protein constructs can be evaluated for relative stability, and an individual stability curve generated for each. Sample preparation, data collection, data analysis and stability report-generation are all automated. The free energy of protein unfolding is automatically calculated for each formulation condition or each individual protein construct.

“Our team’s long experience in life science instrument and systems development and our established relationships with pharmaceutical industry and academic scientists enabled us develop the 2304 and to directly address this important need in the development of safe and effective biologics,” said Rick Brown, president and co-founder of AVIA Biosystems.

In pharmaceutical discovery and development settings, large numbers of constructs or formulations need to be rapidly and easily compared (rank-ordered) for stability. For these applications, automated chemical denaturation provides the most powerful and convenient means to assess protein stability.

The AVIA Biosystems Model 2304 makes, for the first time, protein chemical denaturation available in a completely automated and integrated instrument.

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ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Process ChemistryProcess chemistry is an important stage of drug development for scaling-up drug production or chemical synthesis reactions. It is useful for optimizing economical and efficient drug production. Process chemistry uses reactors and pump systems as well as reagents, standards and buffers.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein StructureProtein structure refers to the three-dimensional arrangement of amino acids in proteins, determining their function. Understanding protein structure is key in drug discovery, enzymology, and molecular biology. Explore protein structure analysis tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.Protein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.

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