Automated Protein Desalting with Bio-Rad’s Profina Protein Purification System

Bio-Rad Laboratories, Inc., a multinational manufacturer and distributor of life science research and clinical diagnostic products, today announced the availability of a new technical bulletin (number 5339A), which compares the desalting of proteins using the Profinia™ protein purification system against a manual desalting method using dialysis.

The bulletin explains how the mechanism of buffer exchange between the most commonly used methods — dialysis and gel filtration chromatography — is not the same. Dialysis will usually produce a more concentrated product than gel filtration, whereas desalting by gel filtration using the Profinia protein purification system, an automated low-pressure chromatography system, offers substantial time savings, an automated scalable process in addition to significant advantages of greatly decreased buffer consumption.

Written by Bio-Rad application scientists, the two-page technical bulletin, provides a comprehensive description of the methods used, a discussion of the results, and a conclusion. The bulletin also includes supporting figures, chromatograms, diagrams, tables, and graphics.

Availability
Bulletin 5539A is available either from a local Bio-Rad sales office or it may be downloaded from the Bio-Rad web site via the article webpage.