Application Note: UPLC® and Online Radioactivity Detection of Metabolites
10 October 2016

The study of the metabolism of a compound plays a key role in drug discovery and optimization. This application note describes the protocol for using the Waters® Open Architecture UPLC® System coupled to a Berthold Radioactivity Monitor LB509 to monitor the metabolites produced when a 3H labelled drug was injected into a hepatocyte suspension culture. The coupling of UPLC® and Online Radioactivity was found to significantly improve the separation and data quality for the identification of the labelled metabolites.