Application Note: Peptide Sample Enrichment Using µPAC™ Trapping Columns
1 April 2019
The online preconcentration of analytes onto low volume trapping columns is a commonly used injection strategy in nano- and microbore LC-MS/MS analysis of complex peptide mixtures. Compared to direct injection onto the analytical column, a sample trapping approach provides several advantages. By effectively desalting and preconcentrating the analytes of interest onto the trap column, analytical column lifetime and workflow throughput can be improved. The trapping configuration allows effective removal of sample matrix components such as salts and contaminants which can interfere with downstream MS analysis, hereby increasing analytical column lifetime and at the same time improving detection sensitivity and the spectral quality that is generated for a sample.