Application Note: Optimization of Trypsin Digestion for MRM Quantification of Therapeutic Proteins in Serum
19 February 2013

In this application note a general workflow for the quantification of therapeutic proteins in serum without analyte pre-fractionation was tested to determine the quantification of trastuzumab in human serum. The analytical workflow of trypsin digestion can be optimized to develop a successful multiple reaction monitoring (MRM) assay. Some of the benefits of trypsin digestion include higher assay sensitivity, reduced sample preparation time, and higher assay reproducibility.