Neurosphere Formation, Differentiation, and Migration of Human Neural Stem Cells Cultured in Corning® Spheroid Microplates

2 May 2017

Neurosphere formation of human neural stem cells (hNSCs) is a widely used in vitro culture system and valuable model to study neurogenesis and neural development. This system allows for three-dimensional (3D) expansion of hNSCs within a more physiologically relevant microenvironment. In this study, Corning spheroid microplates were used for neurosphere formation, proliferation, and migration of hNSCs in an easy-to-use format that is amenable to high throughput screening.

Agilent BioTek Gen5 Software for Detection

Agilent Technologies

Agilent BioTek Gen5 software for Agilent BioTek multimode and single-mode microplate readers is an integrated tool for endpoint, kinetic, spectral scanning, and well area scanning. It controls all the functions of the plate reader and has powerful data analysis capabilities for a broad range of applications. Gen5 microplate reader software is also used to integrate BioTek plate readers to BioTek BioStack and other automated systems.

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Corning® 384-well Black/Clear Round Bottom Ultra-Low Attachment Spheroid Microplate, Bulk Packed 10 per Bag, with Lid, Sterile

Corning Life Sciences

Ultra-Low Attachment microplates feature a covalently bound hydrogel layer that effectively inhibits cellular attachment Surface minimizes protein absorption, enzyme activation and cellular activation Surface is noncytotoxic, biologically inert and nondegradable Sterilized by gamma irradiation Black wall microplates have low background fluorescence, minimal light scatter and reduced crosstalk Opaque walls to prevent well-to-well crosstalk Can be used for both top and bottom reading instruments Bulk packed 10 per bag Novel well geometry aids formation of spheroids in center of well Optically clear round bottom with black opaque microplate body

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Corning® 96-well Black/Clear Round Bottom Ultra-Low Attachment Surface Spheroid Microplate, Bulk Packed, with Lid, Sterile, 10/Bag

Corning Life Sciences

Ultra-low attachment surface with covalently bonded hydrogel that minimizes cell attachment, protein absorption, enzyme activation and cellular activation The surface is noncytotoxic, biologically inert and nondegradable Round bottoms with 300 µL total volume Recommended working volumes of 0.075 to 0.30 mL Sterilized by gamma radiation Novel well geometry aids formation of spheroids in center of well Optically clear round bottom with black opaque microplate body

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