Application Note: Measuring Reactive Oxygen Species with SpectraMax Microplate Readers
25 February 2019
Reactive Oxygen Species (ROS) are chemically reactive molecules containing oxygen. In eukaryotes, these molecules are mainly created during aerobic respiration and can cause problems, such as DNA damage and lipid peroxidation, resulting in cellular damage. ROS are also involved in apoptosis, cellular aging, and cell signaling. Naturally, cells protect themselves from these oxidative species with reducing enzymes and antioxidants, but as cells accumulate more ROS than can be processed, cells will shift towards a state of oxidative stress. Thus, the accurate measurement of cellular ROS levels is very important to understanding the roles ROS play in cellular biology and overall physiology.