Application Note: Malvern Instruments: The Theory and Practice Behind Isothermal Titration Calorimetry
5 August 2015

Isothermal titration calorimetry (ITC) is an analytical technique that has become the “gold standard” for studying intermolecular interactions. As its name indicates, it is a titrimetric technique, that is, a volumetric laboratory method for quantitative chemical analysis (traditionally intended to determine the unknown concentration of an identified analyte) where a reagent solution, the titrant, is made to react with a solution of analyte or titrand. This white paper details the use of ITC in biophysical studies.