Label-Free Phenotype MicroArrays™ Analysis of Cellular Energetics and Apoptotic Activity Using Microplate Reading and Phase Contrast Imaging

20 Apr 2015

Phenotype MicroArrays™ is a unique label-free multiplexing technology for screening cell-based energetics that describe a variety of metabolically related phenotypes in a target cell model. This application note demonstrates a method to combine cellular metabolic Phenotype MicroArrays™ and automated, digital widefield phase contrast microscopy to screen differential nutritional pathway usage of test cells, in addition to potential pathway interruption by an apoptotic compound.

Agilent BioTek Gen5 Software for Detection

Agilent Technologies

Agilent BioTek Gen5 software for Agilent BioTek multimode and single-mode microplate readers is an integrated tool for endpoint, kinetic, spectral scanning, and well area scanning. It controls all the functions of the plate reader and has powerful data analysis capabilities for a broad range of applications. Gen5 microplate reader software is also used to integrate BioTek plate readers to BioTek BioStack and other automated systems.

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Label-Free Phenotype MicroArrays™ Analysis of Cellular Energetics and Apoptotic Activity Using Microplate Reading and Phase Contrast Imaging