Incorporation of a Novel, Automated Scratch Tool and Kinetic Label-Free Imaging to Perform Wound Healing Assays

The wound healing or "scratch" assay is one of the most highly used in vitro methods to monitor and quantify collective cell migration. The current standard involves manual wound creation, which yields low reproducibility between wounds, high variability within generated data, and possible false conclusions regarding test molecules. Using an automated wound creation tool, in addition to kinetic image capture and analysis, repeatable wounds and robust and repeatable results are easily attained.